겨우살이 추출물의 항돌연변이 효과

Antimutagenic Effect of Korean Mistletoe Extracts

  • 함승시 (강원대학교 식품생명공학부) ;
  • 강신태 (강원대학교 식품생명공학부) ;
  • 최근표 (강원대학교 식품생명공학부) ;
  • 박원봉 (서울여자대학교 화학과) ;
  • 이득식 (강원대학교 식품생명공학부)
  • 발행 : 1998.04.01

초록

This study was carried out to investigate mutagenecity and antimutagenic effects from crude extract, heating extract and alcohol extract of Korean mistletoe(Viscum album L.) on the bacterial short-term tests, such as Ames test, spore rec-assay, SOS spot test and SOS chromotest by using several kinds of mutagens. In the Ames test, each extract did not show any mutagenesis, but each extract showed inhibitory effects of 80∼95% and 70∼94% against mutagenesis induced by 3-amino-1, 4-dimethyl-5H-pyrido[4,3-b] indole(Trp-P-1) and 2-aminofluorene(2-AF) in Salmonella typhimurium TA98, respectively. In th spore rec-assay, mistletoe ectracts showed antimutagenic effect with inhibiton zone in the range of 5∼11mm against mutagenicity induced by mitomycin C(MMC, 18mm) and N-methyl-N'-nitro-N-nitrosoguanidne (MNNG, 24mm), respectively. The heating and alcohol extracts in the SOS chromotest showed 96% and 70% inhibition against benzo-α-pyrene[B(α)P] and Trp-P-1 induced mutagenesis, respectively.

키워드

참고문헌

  1. Oncology v.43 no.SUP.1 botany of European mistletoe(Viscum album L.) Becker, H.
  2. Biochem. Biophys. Acta v.104 Amino acid pattern and malignant growth Vester, F.
  3. Physiol. Chem. Hoppe-Seyler v.349 Zur Kenntnis der inhaltsstoffe von Viscum album Ⅲ, isolierung und reinigungcancerostatischer protein fraktionen Vester, F.;Steel, A.;Stoll, M.;Mulle, M.
  4. Geisteswissenschaft und medezin Steiner, R.
  5. Spiritual sciences and medicine Steiner, R.
  6. Biol. Chem. Hoppe-Seyler v.374 Efficient induction of superoxide release from human neutrophils by the galactoside-specific lectin from Viscum album Timoshenko, A. V.;Gabius, H. J.
  7. Oncology v.43 no.SUP.1 Comparison of the effects of fermented and unfermented mistletoe preparations on cultured tumor cells Gayon, G. R.;Jung, M. L.;Scala, D. D.;Beck, J. P.
  8. Cancer Res. v.50 Increased secretion of tumor necrosis factor α, interleukin 6 by human mononuclear cells exposed to β-galactoside-specific lectin from clinically applied mistletoe extract Hajto, T.;Hostanska, K.;Frei, K.;Rordorf, C.;Gabius, H. J.
  9. J. Biol. Regul. Homeost. Agent v.7 Mistletoe(Viscum album) lectins as cytokine inducers and immunoadjuvant in tumor therapy. A review Bocci, V.
  10. 한국약학회지 v.38 유산균 발효에 의한 겨우살이중의 렉틴성분의 변화; 분리 및 정제 박원봉;김희석
  11. 한국생약학회지 v.25 Macrophage의 IL-1 및 TNF-α의 분비유도에 있어서 한국산 겨우살이 추출물이 미치는 영향 윤택준;유영춘;홍은경;조영호;이석원;Azuma, L.;유보림;김종배
  12. 한국약학회지 v.39 유산균 발효에 의한 겨우살이중의 렉틴성분의 변화; pH, 온도의 영향, 당특이성, 림프구 자극분열 효과 박원봉;김희숙;나혜복;함승시
  13. 한국식품과학회지 v.29 가열처리, 당의 첨가 및 발효에 의한 한국산 겨우살이의 세포독성 변화 박종흠;현창기;신현길;여익현
  14. Cancer Lett. v.1 Mutagenicity of carcinogenic azo dyes and their derivatives Yakagi, T.;Degawa, M.;Seino, Y.;Matsushima, T.;Nagao, M.;sugimura, T.;Hashimoto, Y.
  15. Mutation Res. v.16 In vitro and and host-mediated "rec-assay" procedures for screening chemical mutagens; and phloxine, a mutagenic red dye detected Kada, T.;Tuikawa, K.;Sadaie, Y.
  16. Fundermentals in cancer prevention Overlapping of carcinogens and mutagens Sugimura, T.;Sato, S.;Nagao, M.;Matsushima, T.;Seino, Y.;Takeuchi, M.;Kawachi, T.
  17. Mutation Res. v.147 The SOS chromotest, a colorimetric bacterial assay for genotoxins; procedures Quillardet, P.;Hofnung, M.
  18. Mutation Res. v.131 The SOS-function-inducing activity of chemical mutagens in Eschrichia coli Ohta, T.;Nakamura, N.;Moriya, M.;Shirasu, Y.;Kada, T.
  19. Mutation Res. v.203 Evaluation of the SOS chromotest Wilhelm, V. D. H.;Claudia, B.;Rainer, G.;Armin, B.
  20. Experiments in Molecular Genetics Miller, J.
  21. SAS/STAT User Guide(release 6.30 edition) SAS Institute
  22. 한국농화학회지 v.31 산채류 가열즙이 돌연변이 억제작용에 관한 연구 함승시
  23. 강원대학교 석사학위논문 산채류 생즙의 항돌연변이원성에 관한 연구 이재훈
  24. 서울여자대학교 자연과학연구논문집 v.4 겨우살이의 성분분석 김희숙;박원봉;함승시