Korean Journal of Veterinary Pathology (한국수의병리학회지)

Korean Society of Veterinary Pathology (한국수의병리학회)
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Growth and metastasis of human malignant melanoma SK-MEL-2 cell line in SCID mice

  • Choi, Yang-Kyu (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Choi, Jae-Yoon (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Jeon, Hea-Sung (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Won, Young-Suk (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Lee, Chul-Ho (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Yoon, Won-Kee (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Jeong, Kyu-Shik (Korea Research Institute of Bioscience and Biotechnology, KIST) ;
  • Lee, Sang-Koo (Biotech Research Institute, LG Chem. Research Park) ;
  • Hyun, Byung-Hwa (Korea Research Institute of Bioscience and Biotechnology, KIST)
  • Published : 1998.06.01
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Abstract

An in vivo model for human melanoma was established with the growth and metastasis of SK-MEL-2 cells. The tumor was introduced into C.B-17 SCID(severe combined immunodeficiency) mice intraperiotneally subcutaeously and intravenous inoculations. Tumors developed in 100% of mice inoculated subcutaneously and intraeritoneally both at site of inoculation and as metastatic tumor in the liver lungs and diaphragm. With intravenous inoculation 50% of mice showed metastasis in the spleen. Additionally metastatic foci that were not detected either by gross and/or standard histopathologic examination were demonstrated in the spleen and lungs by immunohistochemistry with HMB-45 monoclonal antibody. We conclude that the SCID mouse supports growth and metastasis of human malignant melanoma SK-MEL-2 cells.

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