Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhancement of cis,cis-Muconate Productivity by Overexpression of Catechol 1,2-Dioxygenase in Pseudomonas putida BCM114

  • Kim, Beum-Jun (Department of Chemical Technology, College of Engineering) ;
  • Park, Won-Jae (Department of Chemical Technology, College of Engineering) ;
  • Lee, Eun-Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Park, Cha-Yong (Department of Chemical Technology, College of Engineering)
  • Published : 1998.12.01
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Abstract

For enhancement of cis,cis-muconate productivity from benzoate, catechol 1,2-dioxygenase (C12O) which catalyzes the rate-limiting step (catechol conversion to cis,cis-muconate) was cloned and expressed in recombinant Pseudomonas putida BCM114. At higher benzoate concentrations (more than 15 mM), cis,cis-muconate productivity gradually decreased and unconverted catechol was accumulated up to 10 mM in the cae of wild-type P. putida BM014, whereas cis,cis-muconate productivity continuously increased and catechol was completely transformed to cis,cis-muconate for P. putida BCM114. Specific C12O activity of P. putida BCM114 was about three times higher than that of P. putida BM014, and productivity was enhanced more than two times.

Keywords

References

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