Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effect of Dissolved Oxygen Concentration on the Metabolism of Glucose in Pseudomonas putida BM014

  • Park, Won-Jae (Department of Chemical Technology, College of Engineering, Seoul National University) ;
  • Lee, Eun-Yeol (Department of Food Science and Technology, College of Engineering, Kyungsung University) ;
  • Park, Cha-Yong (Department of Chemical Technology, College of Engineering, Seoul National University)
  • Published : 1998.12.01
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Abstract

The effect of dissolved oxygen concentration on the metabolism of glucose in Pseudomonas putida BM014 was investigated. Glucose was completely converted to 2-ketogluconate via extracellular oxidative pathway and then taken up for cell growth under the condition of sufficient dissolved oxygen concentration. On the other hand, oxygen limitation below dissolved oxygen tension (DOT) value of 20% of air saturation caused the shift of glucose metabolism from the extracellular oxidative pathway to the intracellular phosphorylative pathway. Specific activities of hexokinase and gluconate kinase in intracellular phosphorylation pathway decreased as the DOT increased, while 2-ketogluconokinase activity in extracellular oxidative pathway increased under the same condition. This result can be usefully applied to microbial transformation of glucose to 2-ketogluconate, the synthetic precursor for iso-vitamine C, with almost 100% yield via extracellular oxidation by simple DOT control.

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References

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