Seasonal Expression of OMT Gene in Relation to Lignin biosynthesis in two Poplar Species

종의 포플라수종에서 리그닌생합성에 관계된 OMT유전자의 발현

  • Park, Young-Goo ;
  • Park, Hee Sung ;
  • Choi, Jang Won ;
  • Sul, Ill Whan ;
  • Chung, Il Kyung ;
  • Shin, Dong Ill
  • 박용구 (경북대 임학과) ;
  • 박희성 (대구효성카톨릭대 식물육종학과) ;
  • 최장원 (대구대 자연자원학부) ;
  • 설일환 (경북대 농업과학기술연구소) ;
  • 정일경 (대구효성가톨릭대 원예과학부) ;
  • 신동일 (대구효성카톨릭대 식물육종학과)
  • Published : 1998.08.01

Abstract

We analyzed lignin content and wxpression of OMT gene during growth season in two hybrid poplar species. OMT gene expression was observed mainy in the developing secondary xylem where major quantity of lignin occurs. Lignin content in the xylem tissue increased as plant resumed growth in the spring and reached the highest in the late August. Change in lignin content was concurrent with that of OMT gene expression, indicating OMT is a key enzyme in lignin biosynthesis.

리그닌함량이 감소한 펄프소재의 육성을 위해 OMT유전자와 리그닌생합성 간의 관계를 2종의 포플라 수종을 이용하여 실험하였다. OMT유전자의 발현은 리그닌생합성이 가장 많이 일어나는 developing secondary xylem에서 높앗다. 이 사부조직에서의 리그닌함량의 증가는 봄에 성장을 개시할 때부터 증가하기 시작하여 8월 말 경에 가장 높았다. 이러한 리그닌의 증가는 OMT유전자의 발현 증가와 일치하였으며, 이는 OMT유전자가 리그닌생합성에서 매우 중요한 역할을 함을 나타내는 것이다.

Keywords

References

  1. Biosynthesis and biodegradation of wood components Biosynthesis of lignin Higuchi, T.;T. Higuchi(ed.)
  2. Proc. Natl. Acad. Sci., USA. v.87 Abnormal plant development and down-regulation of phenylpropanoid biosynthesis in transgenic tobacco containing a heterologous phenylalanine ammonia-lyase gene Elkind Y.;Edwards, R.;Mavandad, M.;Hedrick, S. A.;Rivak, O.;Dixon, R. A.;Lamb, C. J.
  3. Plant Physiology v.115 Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamete 4-hydroxylase Sewalt, V. J. H.;Ni, W.;Blount, J. W.;Jung, H. J.;Masoud, S. A.;Howles, P. A.
  4. Plant Mol. Biol. v.17 cDNA cloning, sequence analysis and seasonal expression of lignin bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen Bugos, R. C.;Chiang, V. L. C.;Campbell, W. H.
  5. Plant Mol. Biol. v.26 Modification of lignin biosynthesis in transgenic tobacco through expression of an antisense O-methyltransferase gene from Populus Dwivedi U. N.;Campbell, W. H.;Yu, J.;Datla, R. S. S.;Bugos, R. C.;Chiang, V. L. C.;Podila, G. K.
  6. Plant J. v.6 Manipulation of lignin quality by down-regulation of cinnamyl alcohol dehydrogenase Halpin, C.;Knight, M. E.;Foxon, G. A.;Campbell, M. M.;Boudet, A. M.;Boon, J. J.;Chabbert, B.;Tollier, M. T.;Schuch, M.
  7. Transgen. Res. v.3 Reduced lignin in transgenic plants containing a caffeic acid O-methyltransferase antisense gene Ni, W. T.;Pavia, N. L.;Dixon, R. A.
  8. Proc. Natl. Acad. Sci., USA. v.86 Systemic accumulation of specific mRNAs in response to wounding in poplar trees Parsons, T. J.;De Bradshaw H. Jr.;Gordon, M. P.
  9. Molecular cloning : A laboratory Manual Maniatis, T.;Fritsch, E. F.;Sambrook, J.
  10. Methods in lignin Chemistry The detection of lignin Nakano, J. M.;S. Y. Lin(ed.);C. W. Dence(ed.)
  11. Plant Physiology v.108 Differential expression of two o-methyltranferases in lignin biosynthesis in Zinnia elegans Ye, J. H.;Varner, J. E.
  12. Plant Physiology v.115 Associarion of caffeoyl coenzyme A 3-O-methyltransferase expression with lignifying tissue in several dicot plants Ye, Z. H.