생명과학회지 (Journal of Life Science)

  • 제8권3호
  • /
  • Pages.348-351
  • /
  • 1998
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

잉어 $\beta$-globin 유전자의 염색체상에서의 다형해석

Polymorphism of Carp $\beta$-globin Gene on Chromosome

  • 진덕희 (강릉대학교 해양생명공학부) ;
  • 청목주 (동경수산대학 유전생화학강좌)
  • 발행 : 1998.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Common DNA fragments of the ${\beta}$-globin gene were observed from six races of the adult common carp: Hybrid-Yamato, Japanese wild type, Mirror, Suwa-Yamato, Scale German, and Saku-Yamato. Chromosomal DNAs isolated from the above six races were digested with restriction endonucleased EcoRI and PstI. The digested fragments were transferred onto nitrocellulose filter and hybridized with a probe of carp ${\beta}$-globin cDNA. Molecular sizes of the hybridized DNA fragments digested with EcoRI were 3.6Kb(Kilo base), 4.3Kb and 15Kb in Hybrid-Yamato, Japanese wild type, Mirror, Scale German and Saku-Yamato carp DNAs. In Scale German and Saku-Yamato carp DNAs, two and one more hybridized DNA fragments were observed, respectively. Molecular sizes of the hybridized DNA fragments digested with PstI were 2.2Kb, 6.5Kb, 7.8Kb and 9.2Kb in Hybrid-Yamato, 2.2Kb, 6.5Kb and 9.2Kb in Japanese wild type, 2.2Kb, 6.5Kb, 7.8Kb, and 13Kb in Mirror, 2,2Kb, 5,5Kb, 6.5Kb, 7.8Kb, 9.2Kb and13Kb in Scale German, and 2.2Kb, 5.5Kb, 6.5Kb, 9.2Kb and Saku-Yamato carp DNA. Therefore, depending on carps, three to six DNA fragments were hybridized with ${\beta}$-globin gene probe. Thus it indicated polymorphysm in the globin gene family of carp.

키워드

참고문헌

  1. Cell v.19 Molecular cloning and characterization of the human like globin gene cluster Fritsch, E. F.;Lawn, R. M.;Maniatis, T.
  2. Cell v.21 The structure of a human α-globin pseudogene and its relationshipto α-golbin gene duplication Proudfood, N. J.;Maniatis, T.
  3. J. Mol. Biol. v.180 The -globin gene ; Its long evolutionary history in the β-globin gene family of mammals Goodman, M.;Kop, B. F.;Czelusniak, J.;Weiss, M. L.
  4. Am. J. Hum. Genet. v.51 Functional profile of the human Fetal γ-globin gene upstream promoter region Lin, H. J.;Han, C. T.;Nienhuis, A. W.
  5. Biochim. Biophys. Acta v.783 Cloning and sequence analysis of a cDNA for the α-globin mRNA of carp, Cyprinus carpio Takeshita, S.;Aoki, T.;Fukumaki, Y.;Takagi, Y.
  6. Nippon Suisan Gakkaishi v.58 no.1 Cloning and complete nucleotide sequence of carp α-globin gene Nbota, K.;Aoki, T.
  7. Proceedings of the Third Asian Fisheries Forum no.3 Complete nucleotide sequences of coding and non-coding regions of the carp α-globin gene Miyata, M.;Aoki, T.;V. P. E. Phang(ed.)
  8. Nippon Suisan Gakkaishi v.59 no.3;6;7 Analysis of α-globin gene family of carp. I. α-globin gene structure of Miyata, M.;Hirono, I.;Aoki, T.
  9. J. Mol. Evol. v.38 Tail-to-tail orientation of the atlantic salmon alpha-and beta-globin genes Wagner, A.;Deryckere, F.;McMorrow, T.;Gannon, F.
  10. Fish. Sci. v.60 Cloning and the nucleotide sequence of carp β-globin gene Jin, D. H.;Hirono, I.;Aoki, T.
  11. Fish. Sci. v.61 The symmetrical arrangement of carp α-and β-globin genes Jin, D. H.;Hirono, I.;Aoki, T.
  12. Bull. Natl. Res. Inst. Aquaculture v.7 Comparison of proximate composition among the five races of common carp Shimma, H.;Sato, R.
  13. J. Appl. Ichthyol. v.4 DNA homology of α-globin gene among five races of carp, Cyprinus carpio Aoki, T.;Nobuta, K.;Nishikawa, Y.Shimma, H.
  14. Current Protocols in Molecular Biology Ausubel, F. M.;Brent, R.;Kingston, R. E.;Moore, D. D.;Seidman, J. G.;Smith, J. A.;Struhl, K.
  15. J. Mol. Biol. v.98 Detection of Specific sequences among DNA fragments separated by gel electrophoresis Southern, E.