The korean journal of orthodontics (대한치과교정학회지)
- Volume 28 Issue 6 Serial No. 71
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- Pages.915-926
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- 1998
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- 2234-7518(pISSN)
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- 2005-372X(eISSN)
An Experimental Study on the Effect of Cytoskeletal Changes or Osteoblastic Cell Activities
골모세포유사세포의 cytoskeletal change가 세포 활성에 미치는 영향에 관한 실험적 연구
- Lee, Jeong-Hwa (Department of Orthodontics, Graduate School, Seoul National University) ;
- Nahm, Dong-Seok (Department of Orthodontics, College of Dentistry, Seoul National University)
- Published : 1998.12.01
Abstract
The cytoskeleton has been shown to form a network, connecting the extracelluar matrix via integrin with the nucleus and the cytoplasmic constituents of the cell. It is therefore assumed that the cytoskeleton may mediate signals generated by perturbations originating in the matrix. The purpose of this study is to examine the effect of cytoskeletal change on osteoblastic cell activities. The author cultured osteoblastic cells obtained from neonatal mouse calvaria. The cells were teated with cytochalasin B(CB) or colchicine (COL) at four concentrations for 3 hours and after another 24 hours the conditioned media was collected and assayed for prostaglandin
Cytoskeleton은 세포핵과 세포외 기질을 연결하고 있어서 기질에 가해지는 물리적 힘에 의해 cytoskeletal change가 유도되고 이에 의해 세포의 개조활성이 영향을 받는다고 생각되어 왔다. 본 연구는 골모세포 활성에 대한cytoskeletal change의 역할을 규명하기 위한 것으로서, 신생 백서로부터 조골세포양 세포를 분리, 배양하고 네가지 농도의 cytochalasin B(CB) 또는 colchicine(COL)을 3시간 처리하였다. 다시 배양액을 교환하고 24시간 동안 배양하여 prostaglandin