Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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The Glycopeptide, a Promoter of Thymidine Uptake, from Aloe Vera

  • Yang, Mi-Rim (Department of Chemistry, Pukyong National University) ;
  • Kang, Chun-Geun (Department of Chemistry, Pukyong National University) ;
  • Roh, Yeon-Suk (Department of Chemistry, Pukyong National University) ;
  • Son, Byeng-Wha (Department of Chemistry, Pukyong National University) ;
  • Choi, Hong-Dae (Department of Chemistry, Dongeui University) ;
  • Park, Young-In (Graduate School of Biotechnology, Korea University) ;
  • Lee, Seung-Ki (College of Pharmacy, Seoul National University) ;
  • Choi, Sung-Won (Department of Pharmacology, Seoul National University, College of Medicine) ;
  • Chung, Myung-Hee (Department of Pharmacology, Seoul National University, College of Medicine)
  • Published : 1998.06.01
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Abstract

As a part of search for new biologically active constituents from aloe, we have isolated a glycopeptide, called G1G1M1DI2, from the gel(G1) of Aloe vera. Chemical and spectroscopic evidence indicated that G1G1M1DI2 is a glycopeptide. The molecular weight of G1G1M1DI2 was about 5,500 daltons, and the carbohydrate and protein contents were 20.9% and 32.6%, respectively. Periodate oxidation and enzymic degradation gave peptide moiety and carbohydrate moiety, respectively. Carbohydrate moiety is composed of fucose, galactose, glucose and mannose in a molar ratio of 0.5:2.4;48.8:48.3. Peptide moiety is composed of fifteen amino acids, and glutamic acid and glycine were the major componants. The glycopeptide, G1G1M1DI2, stimulated thymidine uptake of SCC 13 cells about 6.5 times the control. This result suggests that this glycopeptide has a skin cell proliferating activity.

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