조선 전통 메주에서 균독소(Mycotoxin) 측정

Detections of the Mycotoxins on the Korean Traditional Home made Mejus

  • 이상선 (한국교원대학교 대학원 생물과학 및 생물교육학)
  • Lee, Sang-Sun (Major in Biological Science and Education, Graduate School, Korea National University of Education)
  • 발행 : 1998.12.30

초록

우리 나라에 중요한 식품의 원료인 메주에 대한 균독소($Aflatoxin\;B_1$와 Ochratoxin A) 생성 여부를 인위적인 혹은 자연적인 환경에서 조사하였다. 채집된 메주에서 균을 분리하여 접종함으로, 인위적인 환경인 독소생성 배지와 콩을 이용한 배지를 이용하여 균독소 생성을 관찰하였다. 조선 전통메주에서 채집된 메주 균인 불완전 진균들은 몇 몇 균이 독소를 생성하였으나, 멸균된 날 콩에는 다만 $2{\sim}3$개의 분리균들이 $Aflatoxin\;B_1$와 Ochratoxin A를 분비하였다. 그러나, 언급된 두 개의 균독소가 함께 생성하는 분리균은 관찰되지 않았다. 그러나, 조선 전통메주에서는 어떤 채집된 메주에서도 위의 균독소가 $0.01{\sim}100\;ng$ 범위에서 관찰되지 않았다. 이러한 결과로 메주 제조에 관련된 메주 균에서 털곰팡이(Mucor species)가 메주 제조에 초기에 작용한 균으로 전통 메주의 발효와 균독소 생성과 관계에서 중요한 것으로 사료된다.

The mycotoxins, $Aflatoxin\;B_1(B)$ and Ochratoxin A(A), were measured from the various mejus manufactured under the artificial or natural conditions by the indirect competitive ELISA; The various fungi isolated from the Korean traditional home (KTH) made meju collected were observed to produce each mycotoxin mentioned above in the toxin producing broth, but only few in the sterilized cereals of soybean under the artificial conditions. Thus, the isolated fungi were not found to produce both A and B toxins in the artificial conditions. Particularly, the any mycotoxin was not determined at the range of 0.01 to 100 ng per gm of the mejus made under the conditions of KTH widely collected in Korea. The mycotoxins produced by the meju-fermenting fungi were seemed or speculated to be degraded in KTH's mejus under the natural conditions. The species of Mucor involved in the initial stage of fermentation were discussed to be important in the fermentations of KTH mejus.

키워드

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