산욕기(産褥期) 감염(感染) 유발(誘發) 세균(細菌)의 생육(生育)을 억제하는 한약재(漢藥材) 탐색(探索)에 관(關)한 연구(硏究)

A study on screeining of antibacterial herb medicines against puerperal infection-bacteria, Proteus vulgaris, Staphylococcus aures, and Enterococcus faecalis

산욕기(産褥期) 감염(感染)이란 분만후(分娩後) 생식기(生殖器)의 세균(細菌)에 의한 감염(感染)을 의미(意味)하며, 산후(産後) 체온상승(體溫上昇)의 대부분(大部分)은 골반감염(骨盤感染)에 의한 것으로 산욕기(産褥期) 감염(感染)의 중요(重要)한 지표(指標)가 된다. 한의학에서 산후발열(産後發熱)의 병인병기(病因病機)는 주로 사독감염(邪毒感染), 혈어(血瘀), 외감(外感), 혈허(血虛) 등으로 보았으며, 치료는 일반적(一般的)으로 산후질환(産後疾患)에는 허증(虛證)이 많아 발표공리(發表攻裏)를 과도(過度)하게 하지 않고, 기혈(氣血)과 영위(營衛)의 조화(調和)에 위주로 하나, 외감(外感)과 이실증(裏實證)을 소홀(疏忽)히 하면 안된다. 따라서 산후발열(産後發熱)을 야기(惹起)하는 각종(各種) 원인균(原因菌)에 대(對)한 객관적(客觀的)인 억제효과(抑制效果)의 검증(檢證)은 산후발열(産後發熱)의 치료율(治療率)을 높이는데 중요하다. 그 결과 산욕기감염을 유발하는 각종(各種) 세균(細菌)에 대(對)해 황연(黃連), 오매(烏梅), 오미자(五味子) 등의 수용성(水溶性) 졸출물(拙出物)이 항균활성(抗菌活性)이 상대적(相對的)으로 좋은 효과(效果)를 보였다. 따라서 산욕기(産褥期) 감염(感染)의 치료(治療)에 이들 한약재(韓藥材)를 이용하면 보다 효과적(效果的)일 것으로 생각된다.

Various kinds of medicinal herbs and prescribed herb medicines which have usually been used for treatment of reducing fever, purging intense heat, and detoxication were secreened for antibacterial activity against Proteus vulgaris, S. aureus, and E. faecalis which causing puerperal infection. Minimal inhibitory concentrations of water and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) were determined and using thin-layer chromatography the extracts of Hwangyon and Omiza were separated. The results obtained were as follow : 1. The water-soluble extracts of Keumeunhwa(L. japonica), Chiza(G. jasminoides), Hwangyon(C. japonica), Dansam(S. miltiorrhiza), Omae(P. mume) and Omiza(S. chinensis) showed antibacterial activities against P. vulgaris ; among them, the extracts of Hwangyon, Omae, and Omiza showed high antibacterial activities. The water-soluble extracts of Keumeunhwa(L. japonica), Hwangyon(C. japonica), Hwangkeum(S. baicalensis), Hwangbaek(P. amurense), Dansam(S. miltiorrhiza), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), Gingseng(P. ginseng), and Gamcho(G. uralensis) showed antibacterial activities against S. aureus : among them, the extracts of Hwangyon and Omiza showed high antibacterial activities. The water-soluble extracts of Banggi(C. trilobus), Daehwang(R. coreanum), Omae(P. mume), Omiza(S. chinensis), and Gamcho(G. uralensis) inhibited the cell growth of E. faecalis : among them, Omae and Omiza showed high antibacterial activities. The extract of Hwangyon(C. Japonica) and Omae(P. mume) showed no inhibition against E. coli. However, the extract of Omiza(S. chinensis) inhibited cell growth of both E. coli and B. subtilis. 2. The water- and ethanol-soluble extract of Hwangyon(C. Japonica) showed antibacterial activities against P. vulgaris and S. aureus, those of Omae(P. mume) against P. vulgaris and E. faecalis, and those of Omiza(S. chinensis) against all species tested, P. vulgaris, S. aureus, and E. faecalis. With the exception that ethanol-soluble extract of Hwangyon(C. japonica) showed much higher antibacterial activity against S. aureus than water-soluble one, antibacterial activities of both water-and ethanol-soluble ones were similar to each other of other two medicinal herbs. 3. When the prescribed herb medicines were tested, Sambohwan showed antibacterial activities against P. vulgaris and S. aureus. No prescribed herb medicine inhibitied the cell growth of E. faecalis. 4. Minimal inhibitory concentrations(MICs) of both water- and ethanol-soluble extracts of Hwangyon(C. japonica), Omae(P. mume), and Omiza(S. chinensis) against P. vulgaris were $2.5mg/m{\ell}$, $10mm{\ell}$, and $20mg/m{\ell}$, respectively. Those of both Hwangyon(C. japonica) and Omiza(S. chinensis) against S. aureus were $1.25mg/m{\ell}$ and $10mg/m{\ell}$, respectively. MICs of water-soluble extracts of Omae(P. mume) and Omiza(S. chinensis) against E. faecalis were $2.5m{\ell}$ and $5m{\ell}$, and those of ethanol-soluble extracts were $5m{\ell}$ and $10m{\ell}$, respectively Except for those of E. faecalis the cell growth of P. vulgaris and S. aureus were inhibited by much lower concentration of ethanol-soluble extracts used. As a result, the antibacterial compounds against P. vulgaris S. aureus, and E. aecalis are contained in the extracts of Hwangyon(C. japonica), Omae(P. mume), Omiza(S. chinensis), the prescribed herb medicine, Sambohwan, and might be used for treatment of puerperal infection. Further study should be carried out to identify which compounds affect the cell growth inhibition of P. vulgaris S. aureus, and E. faecalis.