Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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The effects of a combination of calcium sulfate and platelet-derived growth factor on periodontal ligament cells in vitro

Calcium sulfate와 혈소판 유래성장인자의 혼합사용이 치주인대세포에 미치는 영향

  • Kim, Jun-Seong (Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration) ;
  • Choi, Seong-Ho (Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration) ;
  • Yu, Yun-Jung (Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Chai, Jung-Kiu (Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration) ;
  • Kim, Chong-Kwan (Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration) ;
  • Cho, Kyoo-Sung (Department of Periodontology, College of Dentistry, Yonsei University, Research Institute for Periodontal Regeneration)
  • 김준성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 최성호 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 유윤정 (연세대학교 치과대학 구강생물학교실) ;
  • 채중규 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 김종관 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소) ;
  • 조규성 (연세대학교 치과대학 치주과학교실, 치주조직재생연구소)
  • Published : 1997.12.31
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Abstract

It was well known that calcium sulfate was biocompatible, resorbed rapidly in the body, had potential as a good barrier membrane. Platelet-derived growth factor(PDGF) was one of polypeptide growth factor that had been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purpose of this study was to evaluate the effects of a combination of calcium sulfate and PDGF on periodontal ligament cells in vitro to use as a regeneration promoting agent of periodontal tissue. Human periodontal ligament cells were prepared from the premolar tooth extracted for the orthodontic treatment. Cells were cultured in ${\alpha}-MEM$ contained with 20% FBS, at the $37^{\circ}C$, 100% of humidity, 5% $Co_2$ incubator. Cells were inoculated and cultured into 96 well culture plate with $1{\times}10^4cells/well$ of ${\alpha}-MEM$ for 1 day. After discarding the medium, those cells were cultured in ${\alpha}-MEM$ contained with 10% FBS alone(control group), in calcium sulfate(calcium sulfate group), in calcium sulfate treated with 15ng/ml of PDGF-BB(calcium sulfate+PDGF group), in ${\alpha}-MEM$ contained with 10% FBS treated with 15ng/ml of PDGF-BB(PDGF group) for 1, 2, 3 day respectively. And then each group was characterized by examining of the cell counting, MTT assay, collagen synthesis. The results were as follows. 1. In the analysis of cell proliferation by cell counting, both calcium sulfate group and calcium sulfate plus PDGF group showed no stastically significant difference compared to control group, but there was stastically significant difference between PDGF group and calcium sulfate group at 1, 2 day(P<0.05). 2. In the analysis of cell proliferation by MTT assay in calcium sulfate extracts, both calcium sulfate group and calcium sulfate plus PDGF group showed no stastically significant difference compared to control group, but there was stastically significant difference between PDGF group and calcium sulfate group at 2, 3 day, and between calcium sulfate plus PDGF group and calcium sulfate group at 2 day(P

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