Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation of a petunia cell growth inhibitor from Streptomyces sp. 9602

Streptomyces sp. 9602 균주로부터 페튜니아 캘러스 생장억제물질의 분리

  • 김명조 (KIST 생명공학연구소 식물생화학 Research Unit) ;
  • 곽상수 (KIST 생명공학연구소 식물생화학 Research Unit)
  • Published : 1997.05.01
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Abstract

To search for a compound inhibiting the petunia callus growth from Streptomyces sp., we investigated the activity in the culture broth of 400 strains. The active compound was successively purified with solvent fractionation, silica gel column chromatography from Streptomyces sp. 9602 strain, and identified as 2, 5, 7-trihydroxy-3-(5'-hydroxyhexyl)-1, 4-naphthoquinone by 1H-NMR, EI-MS, IR and UV. It inhibited the callus growth of petunia by 50% at $32\mu\textrm{g}$/mL.

토양 방선균(Streptomyces sp.)으로부터 페튜니아 캘러스의 생장을 억제하는 물질을 탐색하기 위하여 400 균주의 배양액을 대상으로 활성을 조사하였다. 방선균 9602 균주의 배양액으로부터 캘러스 생장억제물질을 용매분획과 2차의 실리카겔 칼람 크로마토그라피를 사용하여 활성물질을 분리하였다. 활성물질은 1H-NMR, EI-MS, IR, UV 등을 사용하여 2,5,7-trihydroxy-3-(5'-hydroxyhexyl)-1,4-naphthoquinone(3-OH NQ)으로 동정하였다. 3-OH NQ는 페튜니아 캘러스 생장을 $32\mu\textrm{g}$/mL 농도에서 50% 억제하였다.

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