Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of pH, Sucrose and Vitamins on the Growth and Tropane Alkaloid Production of Hairy Roots of Datura stramonium var. tatula Torr.

독말풀(Datura stramonium var. tatula Torr.) 모상근의 성장과 tropane alkaloid 생성에 미치는 pH, 서당 및 비타민의 영향

  • 양덕조 (충북대학교 자연과학대학 생명과학부) ;
  • 강현미 (충북대학교 자연과학대학 생명과학부) ;
  • 이강섭 (충북대학교 자연과학대학 생명과학부) ;
  • 김용해 (충북대학교 자연과학대학 생명과학부) ;
  • 양덕춘 (한국인삼연초연구원)
  • Published : 1997.05.01
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Abstract

The optimum concentrations of pH, sucrose and vitamins for the growth and tropane alkaloid production of hairy root clone DTLA9 (best growth line) were investigated. The optimum pH in growth and tropane alkaliod production of DTLA9 clone in SH (Schenk and Hildebrandt, 1972) basal medium without growth regulator were pH 6.3 and 6.5, respectively. Also, the optimum sucrose concentration in growth and tropane alkaliod production in the same medium were 3.0 and 2.8%, respectively. The optimum concentrations of ascorbic acid, D-pantothenate, nicotinic acid, pyridoxine, riboflavin, and thiamine on the growth of DTLA9 clone in SH basal medium without vitamins were 0.1 mM, 0.003 mM, 0.07 mM, 0.002 mM, 0.025 mM, and 0.01 mM, respectively. In particular, supplement of 0.1 mM ascorbic acid to SH basal medium without vitamins stimulated the tropane alkaloid production.

모상근의 성장 및 tropane alkaloids의 생성에 미치는 pH, 서당, 비타민의 영향을 구명하기 위하여, 독말풀(Datura stramonium var. tatula Torr.)의 잎에 Agrobacterium tumefaciens $A_4$T를 접종하여 모상근을 유도하였다. 유도된 모상근 중 성장률이 양호한 clone (DTLA9)을 선발하고, 이를 pH, 서당, 비타민을 각각 여러 농도로 처리한 SH(Schenk and Hildebrandt, 1972) 기본배지에 배양하였다. 모상근의 성장에 있어 최적 pH는 6.3이었으며, 최적 서당농도는 3.0%이었다. Tropane alkaloids의 함량에 있어 최적 PH는 6.5이었으며, 최적 서당농도는 2.8%이었다. 한편, 비타민이 제거된 SH기본배지에 ascorbic acid, D-pantothenate, nicotinic acid, pyridoxine, riboflavin. 그리고 thiamine을 각각 농도별로 첨가하여 배양한 경우에, 모상근의 성장에 있어 비타민의 최적 농도는 각각 0.1, 0.003, 0.07, 0.002, 0.025, 0.01 mM이었다. Tropane alkaloids의 함량은 0.1 mM의 ascorbic acid 단독처리구에서 대조구(vitamin-free구) 및 SH기본배지에서의 경우에 비하여 가장 높게 나타났다.

Keywords

References

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