Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Expression of Mouse Synaptobrevin (VAMP) Gene in E. coli and its Cleavage by the Clostridium botulinum type B Toxin

Synaptobrevin (VAMP)유전자의 대장균에서의 발현 및 Clostridium botulinum type B 독소에 의한 절단

  • 정현호 (선문대학교 자연과학대학 미생물학과) ;
  • 양기혁 (한국과학기술원 생명과학과) ;
  • 이상달 (한국과학기술원 생명과학과) ;
  • 양규환 (한국과학기술원 생명과학과)
  • Published : 1997.12.01
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Abstract

Synaptobrevin is a kind of vesicle associated membrane proteins (VAMPs) which plays a secretary role in the neuronal synapse and was recently known as the biochemical target of botulinum neurotoxin type B. The structural gene of the synaptobrevin was cloned from mouse brain using RT-PCR technique and was seqrtenced. The deduced amino acid sequence showed that the synaptobrevin protein from mouse brain is exactly the same with that of the rat brain in the amino acid level. The synaptobrevin gene was subcloned into pET3a vector and expressed in E. coli. The molecular weight of the recombinant protein was 19 kDa as expected. Moreover, when the recombinant synaptobrevin protein was incubated with the native neurotoxin of Clostridium botulinum type B, it was cleaved by the toxin in a time dependent manner. This implies that the recombinant synaptobrevin protein and the native toxin are reacted in the same way as the native synaptobrevin did in the neuronal cells.

Keywords

References

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