The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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A Study for the isolation of the Berberine-type Alkaloid from Coptidis Rhizoma and for their Antitumor Activities

  • Shin, Kwhang-Ho (Department of Phytology, College of Oriental Medicine, Kyung Hee University) ;
  • Ahn, Duk-Kyun (Department of Phytology, College of Oriental Medicine, Kyung Hee University) ;
  • Woo, Eun-Ran (Division of Applied Science, Korea Institute of Science and Technology) ;
  • Lee, Eun-Ju (Advanced Analysis Center, Korea Institute of Science and Technology) ;
  • Rhee, Jae-Seong (Advanced Analysis Center, Korea Institute of Science and Technology)
  • Published : 1997.04.30
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Abstract

The purpose of this study is the separation of biologically active ingredients from Coptidis Rhizoma which has been widely used as one of oriental herbal medicine for body fever. In this study, berberine-type alkaloids were tested on their biological activities in the aspect of antibacterial, antitumor, anti-herpetic and anti-HIV activity. Contents of five major alkaloids for the various origin of Coptidis Rhizoma were assayed by HPLC. As the results, the content of berberine from Coptis chinensis and Coptis japonica were 6.78% and 7.09%, respectively. The contents of coptisine, jatrorrhizine, berberastine from Coptis chinensis were higher than those of Coptis japonica. The amount of palmatine from both species were almost the same. Surprisingly for antitumor experiment, all compounds have been shown remarkable activity, especially against SNU-l(human stomach cancer) cell line. Among the compounds purified through column chromatography, palmatine, coptisine, and jatrorrhizine inhibited the growth of K-562(human chronic myelogenous leukemia) cell line whereas jatrorrhizine has been shown the effective inhibition of A-549 (human lung) cell line at the same time.

Keywords

References

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