두부공업폐수에서 Rhodobacter capsulatus KK-10을 이용한 ${\delta}-Aminolevulinic$ Acid의 생산

Production of ${\delta}-Aminolevulinic$ Acid in Soybean Curd Wastewater by Rhodobacter capsulatus KK-10

광합성세균을 이용한 고농도 유기폐수인 두부공업폐수의 정화처리와 동시에 두부공업폐수를 생물제초제인 ${\delta}-aminolevulinic$ acid (ALA) 생산용 대체기질로 이용할 목적으로 Rhodobacter capsulatus KK-10을 사용하여 COD 제거율과 ALA 생산능을 검토하였다. 사용된 두부공업폐수의 성상은 pH 4.2, COD/BOD와 BOD/N의 비율은 0.98 및 17.2, BOD : N : P의 함유비율은 10 : 6 : 4, 젖산 함유량 1,080 ppm 등으로 광합성세균의 생육에 적당한 조건이었다. 두부공업폐수에서 본 균주를 4일 동안 배양한 결과 약 94% COD 값의 감소와 1.2 g/l 균체생육을 보였으며 대수기 중기에서 ALA 탈수효소 저해제인 levulinic acid(LA)를 15 mM 첨가한 결과 55 mg/l의 ALA 생산량을 나타내었다. ALA의 전구물질인 succinate 및 glycine과 LA를 각각 15 mM의 농도로 동시 첨가시 최대 114 mg/l의 생산량을 보였다. 상기의 물질들을 각각 15 mM 농도로 연속 첨가한 후 ALA 생성량을 검토한 결과 LA 1회와 glycine과 succinate를 3회에 걸쳐 첨가하였을 때 120 mg/l로 가장 효과적이었으며 COD 제거율은 92%를 나타내었다.

The removal efficiency of COD and the production of ${\delta}-aminolevulinic$ acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P=100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/l after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 mg/l. The ALA production was considerably increased to 114 mg/l under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 mg/l and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.