YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Isolation and Characterization of Soil Microorganism Producing Acyl CoA Synthetase Inhibitor

Acyl CoA 합성효소 억제제를 생산하는 토양균의 분리 및 특성 연구

  • Kim, Kyoung-Ja (Department of Life Science, Soonchunhyang University) ;
  • Kim, Tae-Sung (Department of Life Science, Soonchunhyang University)
  • 김경자 (순천향 대학교 자연과학대학 생명과학부) ;
  • 김태성 (순천향 대학교 자연과학대학 생명과학부)
  • Published : 1996.12.01
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Abstract

Identification of soil microorganism strain B-6. a producer of acyl CoA synthetase inhibitor, based on its morphological, physiological, biochemical and chemotaxonomical charact eristics was performed. The strain B-6 was identified as Bacillus subtilis. Ihe acyl CoA synthetase inhibitor produced by this strain was highly achieved in fermentation medium that contained glucose 1.0%, soluble starch 1.0%, NH$_4$Cl 0.3%, oatmeal 1.0%, pharmamedia 1.0%, basic magnesium carbonate 0.5%. pH 7.5 at 30$^{\circ}$C for 7 days. The optimal pH and temperature for growth were 9.0 and 30$^{\circ}$C, respectively. Butanol extract of culture filterate of strain B-6 in acyl CoA synthetase inhibitor production medium containing corn steep liquor exhibited high acyl CoA synthetase inhibitor activity and antimicrobial activity against C. albicans. But chloroform extract of culture filterate of strain B-6 in medium containing NH$_4$Cl, ($NH_4)_2SO_4$ or urea instead of corn steep liquor exhibited higher antimicrobial activity against C. albicans than that of butanol extract.

Keywords

References

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