Journal of Nutrition and Health

  • 제29권9호
  • /
  • Pages.971-980
  • /
  • 1996
  • /
  • 2288-3886(pISSN)
  • /
  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

사람 혈청 트란스페린수용체의 단클론 항체를 이용한 방사면역측정과 철영양상태의 진단

Immunoradiometric Assay using Monoclonal Antibody Against Human Serum Transferrin Receptor for Diagnosis of Iron Deficiency

  • 김승렬 (서울대학교 의과대학 생화학교실)
  • 발행 : 1996.11.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The soluble transferrin receptor(TfR) in human serum has been shown recently to be a truncated form of intact membrane bound receptor containing most of the extracellular domain. We purfied the transferin-free TfR from human serum by immounoaffinity chromatography which produced the single protein identity in high resolution gel chormatography. The monoclonal antibodies(MAb) against purifed serum TfR were produced by fusion of spleen cells o fimmunized Balb/c mice and SP2 cells. Ten hybrids producing MAb specific for serum TfR were identifed and determine their iostypes. A immunoraddiometric assay (IRMA) for serum TfR was established using two monoclonal IgG1 antibodies as the coating and indicator antibodies on the bosis of their suitability in sandwich IRMA of serum TfR. The mean serum TfR levels in the 15 normal male, 15 normal female, and 19 iron-deficient subjects were 5.4$\pm$0.98, 4.6$\pm$0/76, and 18.0$\pm$12.8mg/1, respectively, and the difference in mean values between normal and iron deficient subjects was significant(p=0.0005). There existed the inverse logarithmic relationship(r=-0.9336, p<0.0001) between the serum TfR and ferritin levels.

키워드

참고문헌

  1. Biochem Pharmacol v.33 Transferrin receptors : Structure and function Trowbridge,I.S.;Newman,R.A.;Domingo,D.L.;Sauvage,C.
  2. Physiol Rev v.67 The physiology of transferrin and transferrin receptors Huebers,H.A.;Finch,C.A.
  3. Invest Radiol v.22 The structure, function and regulation of transferrin receptors Ward,J.H.
  4. J Clin Invest v.42 The plasma-to-cell cycle of transferrin Jandl,J.H.;Katz,J.H.
  5. J Clin Pathol v.36 Transferrin receptors in human tissues : their distribution and possible clinical relevance Gatter,K.C.;Brown,G.Y.;Trowbridge,I.S.;Woolston,R.E.;Mason,D.Y.
  6. Nature v.311 Primary structure of human transferrin receptor deduced from the mRNA sequence Schneider,C.;Owen,M.J.;Banville,D.;Williams,J.G.
  7. Cell v.39 The human transferrin receptor gene : Genomic organization and the complete primary structure of the receptor deduced from a cDNA sequence McClelland,A.;Kuhn,L.C.;Ruddle,F.H.
  8. Br J Haematol v.64 Circulating transferrin receptor in human serum Kohgo,Y.;Nishisato,T.;Kondo,H.;Tsushima,N.;Niitsu,Y.;Urushizaki,I.
  9. Blood v.70 Serum transferrin receptor as a new index of erythropoiesis Kohgo,Y.;Niitsu,Y.;Kondo,H.;Kato,J.;Tsushima,N.;Sasaki,K.;Hirayama,M.;Numata,T.;Nishisato,T.;Urushizaki,I.
  10. Jpn J Med v.27 Quantitation and characterization of serum transferrin receptor in patients with anemias and polycythemias Kohgo,Y.;Niitsu,Y.;Nishisato,T.;Kato,J.;Kondo,H.;Tsushima,N.;Urushizaki,I.
  11. J Lab Clin Med v.114 The clinical measurement of serum transferrin receptor Flowers,C.H.;Skikne,B.S.;Covell,A.M.;Cook,J.D.
  12. Blood v.75 Intact transferrin receptors in human plasma and their relation to erythropoiesis Huebers,H.A.;Beguin,Y.;Pootrakul,P.;Einspahr,D.;Finch,C.A.
  13. Blood v.75 Serum transferrin receptor : A quantitative measure of tissue iron deficiency Skikne,B.S.;Flowers,C.H.;Cook,J.D.
  14. Pro Soc Exp Biol Med v.197 Characterization of transferrin receptor released by K562 erythroleukemia cells Baynes,R.D.;Shih,Y.J.;Hudson,B.G.;Cook,J.D.
  15. Blood v.59 A monoclonal antibody that detects expression of transferrin receptor in human erythroid precursor cells Lebman,D.;Trucco,M.;Bottero,L.;Lange,B.;Pessano,S.;Rovera,G.
  16. Cancer Res v.43 Molecular identification of the TCA alloantigen as the transferrin receptor on T-cells of a leukemic patient with serum from a monozygotic twin brother Derks,J.P.A.;Hofmans,L.;Bruning,H.W.;Van Rood J.J.
  17. J Lab Clin Med v.114 Immunoassay of serum transferrin receptors : Clinical implications Trowbridge,I.S.
  18. J Biol Chem v.265 Serum transferrin receptor is a truncated form of tissue receptor Shih,Y.J.;Baynes,R.D.;Hudson,B.G.;Flowers,C.H.;Skikne,B.S.;Cook,J.D.
  19. Blood v.81 Characterization and quantitation of the circulating forms of serum transferrin receptor using domain-specific antibodies Shih,Y.J.;Baynes,R.D.;Hudson,B.G.;Cook,J.D.
  20. J Biol Chem v.263 A high yield purification of the human transferrin receptor and properties of its major extracellular fragment Turkewitz,A.P.;Amatruda,J.F.;Borhani,D.;Harrison,S.C.;Schwartz,A.L.
  21. J Biol Chem v.256 Receptor-mediated endocytosis of transferrin in developmentally totipotent mouse teratocarcinoma stem cells Karin,M.;Mintz,B.
  22. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgrm quantities of protein utilizing the principle of protein-dye binding Bradford,M.M.
  23. Gel Electrophpresis of Proteins : A Practical Approach Hames,B.D.;Hames,B.D.(ed.);Rickwood,D.(ed.)
  24. Immunochemistry in Practice (1st ed.) Johnstone,A.;Thorpe,R.
  25. J Histochem Cytochem v.22 Peroxidase-labeled antibody : A new method of conjugation Nakane,P.K.;Kawaoi,A.
  26. Can J Biochem v.58 Reticulocyte membrane transferrin receptors Ecarot-Charrier,B.;Grey,V.L.;Wilczynska,A.;Schulman,H.M.
  27. Nature v.256 Continuous cultures of fused cells secreting antibody of predefined specificity Khler,G.;Milstein,C.
  28. Lancet v.2 Iron deficiency without anaemia Fielding,J.;O'Shaughnessy,M.C.;Brunstrom,G.M.
  29. Am J Clin Nutr v.27 Serum ferritin as a measure of iron stores in normal subjects Cook,J.D.;Lipschitz,D.A.;Miles,L.E.M.;Finch,C.A.
  30. J Cell Physiol v.122 Regulation of K562 cell transferrin receptors by exogenous iron Rudolph,N.S.;Ohlsson-Wilhelm B.M.;Leary,J.F.;Rowley,P.T.
  31. J Biol Chem v.260 Hemin, chelatable iron, and the regulation of transferrin receptor biosynthesis Rouault,T.;Rao,K.;Harford,J.;Mattia,E.;Klausner,R.D.
  32. Proc Natl Acad Sci USA v.85 Transferrin receptors in rat plasma Beguin,Y.;Huebers,H.A.;Josephson,B.;Finch,C.A.
  33. J Lab Clin Med v.19 Serum transferrin receptor distinguishes the anemia of chronic disease from iron deficiency anemia Ferguson,B.J.;Skikne,B.S.;Simpson,K.M.;Baynes,R.D.;Cook,J.D.
  34. Scand J Immunol v.20 Monoclonal antibodies to a purified human transferrin receptor Nikinmmaa,B.;Enns,C.A.;Tonik,S.E.;Sussman,H.H.;Schroder,J.
  35. Proc Natl Acad Sci USA v.79 Monoclonal antibody to transferrin receptor blocks transferrin binding and inhibits human tumor cell growth in vitro Trowbridge,I.S.;Lopez,F.
  36. Pro Soc Exp Biol Med v.204 Production of the serum form of the transferrin receptor by a cell membrane-associated serine protease Baynes,R.D.;Shih,Y.J.;Hudson,B.G.;Cook,J.D.