KSBB Journal
- Volume 11 Issue 4
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- Pages.504-509
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- 1996
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
Screening of Promoter Sequences from Lactic Acid Bacteria Using a Promoter-Selection Vector
Promoter-Selection Vector를 사용한 유산균 Promoter의 탐색
Abstract
Promoters which are useful for constructing expression vectors for lactic acid bacteria were obtained from the chromosomal DNA of Lactococcus lactis ssp. lactis MG1363. pBV5030, a promoter-selection vector, replicates in L. lactis and Escherichia coli and carries a promoterless chloramphenicol acetyltransferase gene (cat-86). After examining E. coli transformants which grew on LB media containing chloramphenicol (Cm, 20
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