Effects of pH and Nitrogen sources on the Pullulan Production by Aureobasidium pullulans

Aureobasidium pullulans에 의한 플루란 생산에서 pH와 질소원의 영향

  • Na, Kun (Department of Chemical Tech., Chonnam National University) ;
  • Lee, Ki-Young (Department of Biochemical Eng., Chonnam National University) ;
  • Park, Don-Hee (Department of Biochemical Eng., Chonnam National University)
  • Published : 1996.08.01

Abstract

In this study, the effects of nitrogen sources and pH on pullulan production were investigated. As a result, the best nitrogen source in pullulan production by Aureobasidium pullulans was shown to be peptone and its product yield was 62%. Optimum concentration of carbon/nitrogen source ratio was 50/0.15 and the production of pullulan was inhibited at ratios higher than 50/0.15. Aureobasidium pullulans had produced 29.1, 27.4 and 26.5 g/L pullulan, respectively in media I, II, and III containing mixed nitrogen sources. This result showed that pullulan could be produced efficiently from mixed nitrogen source. It was found that pullulan yield with pH control was higher than that with no pH control. In fedbatch fermentation, pullulan yield obtained with a feeding rate of 0.05 N-g/L for nitrogen source was higher than that without nitrogen source feeding.

본 연구에서는 질소원의 종류와 농도 그리고 pH가 플루란 생산에 미치는 영향을 고찰하였다. A Aureobasidium pullulans에 의한 플루란 생산시 가장 좋은 질소원으로는 수율 62%를 얻은 peptone 이었고, 최적 질소원 농도는 유기질소원의 경우 탄 소원/질소의 비가 50j0.15N이었고 그 이상에서는 저해가 일어났다. 훈합질소원 I, II, III을 사용하 였을 때 각각 29.1, 27.4, 26.5g/L의 플루란 생산량 을 보였다. 그러므로, 혼합 질소원을 잘 이용하면 플 루란을 효과적으로 생산할 수 있었다. pH를 조절하 지 않을 때가 pH를 일정하게 할 때보다는 플루란 수율이 높았다. 또, 유가식배양시 탄소원과 질소원을 함께 공급하였을 때가 질소원만을 공급하였을 때 더 큰 플루란 수율을 보였다.

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