Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Analysis of Telopeptide Removal in Type I Collagen Purified From Human Umbilical Cords

사람 탯줄로부터 추출된 Type I Collagen의 Telopeptide 제거에 대한 분석

  • Suh, Hwal (Department of Medical Engineering, Yonsei University College of Medicine) ;
  • Ahn, Sue-Jin (Biomedical Research Center, Korea Institute of Science and Technology) ;
  • Kim, Yo-Sook (Department of Biology, College of Natural Science, the Catholic University of Korea) ;
  • Lee, Ha-Gyui (Department of Biology, College of Natural Science, the Catholic University of Korea)
  • 서활 (연세대학교 의과대학 의용공학교실) ;
  • 안수진 (한국과학기술연구원 의과학연구센터) ;
  • 김요숙 (카톨릭대학교 자연대학 생물학과) ;
  • 이하규 (카톨릭대학교 자연대학 생물학과)
  • Published : 1996.09.01
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Abstract

Although collagen is still considered to be a poor immunogen, animals can produce antibodies to a number of different sites in the collagen molecule. In type I collagen, three classes of antigenic determinants have been described those are recogrlized as different degrees in different species. These are essentially composed of helical, conformation-dependent antigenic determinants and terminal, nonhelical antigenic determinants, and finally central antigenic determinants exposed only after denaturation of the collagen molecule. To utilize collagen as implantable biomateriall human e61bryonic collagen, ten immunological to body, was purified from human umbilical cords and found to contain [$\alpha$1(I)]$_2$. [$\alpha$2(I). Each step of purification were observed by polarized light microscope and analyzed through SDS-PAGE. The conclusious are follows; 1 . The purified collagen revealed gradual fiber indenties on each step of purification by polarized microscope. 2. The structual changes of extracted collagen as removed telopeptide were confirmed by SDS-PAGE.

Keywords

References

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