The Antioxidant Activity of Ecklonia stolonifera

  • Lee, Ji-Hyeon (Dept. of Nutrition and Food Sceience, National Fisheries University of Pusan) ;
  • Park, Jong-Cheol (Dept. of Oriental Medicine Resources, Sunchon National University) ;
  • Choi, Jae-Sue (Dept. of Nutrition and Food Sceience, National Fisheries University of Pusan)
  • Published : 1996.06.01

Abstract

The antioxidant activity of Ecklonia stolonifera was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$ using 2-thiobarbituric acid (TBA) and the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The methanol extract of Ecklonia stolonifera showed strong antioxidant activity. And the methanol extract was fractionated with several solvents. With regard their fractions, the antioxidative activity were in the order of ethyl acetate>dichloromethane insoluble intermediated phase>dichloromethane>n-butanol>water fraction. The ethyl acetate soluble fraction exhibiting the strongest antioxidant activity was further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant phloroglucinol was isolated and identified by$ ^1H-NMR\; and\; ^{13}C-NMR$. Its antioxidant activity was simlilar to that of L-ascorbic acid.

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