Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Studies on Development of New Basidiomycetes by Protoplast Fusion and Nuclear Transfer I - The Antitumor Components of the Protoplast fusants -

원형질체 융합 및 핵전이에 의한 새로운 담자균류의 개발에 관한 연구(I) - 융합균사체의 항암성분 -

  • Moon, Chul (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Yoon, Jong-Myung (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Kim, Chae-Kyun (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Kim, Ha-Won (Seoul City University) ;
  • Choi, Eung-Chil (Department of Microbial Chemistry, College of Pharmacy, Seoul National University) ;
  • Kim, Byong-Kak (Department of Microbial Chemistry, College of Pharmacy, Seoul National University)
  • 문철 (서울대학교 약학대학 미생물약품화학교실) ;
  • 윤종명 (서울대학교 약학대학 미생물약품화학교실) ;
  • 김채균 (서울대학교 약학대학 미생물약품화학교실) ;
  • 김하원 (서울시립대학교) ;
  • 최응칠 (서울대학교 약학대학 미생물약품화학교실) ;
  • 김병각 (서울대학교 약학대학 미생물약품화학교실)
  • Published : 1996.09.30
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Abstract

To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.

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