The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Biological and Physico-chemical Properties of Antifungal Cyclic Lipopeptides Produced by Pseudomonas cepacia Strains

Pseudomonas cepacia 균주가 생산하는 항진균성 Cyclic Lipopeptide의 생물학적 및 물리 화학적 특성

  • 김성호 (동국대학교 응용생물학과) ;
  • 이민웅 (동국대학교 응용생물학과)
  • Published : 1996.12.30
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Abstract

Five strains AF027, AF069, AF2001, AF2011 and SD02 of Pseudomonas cepacia were isolated from soil, and the antifungal cyclic lipopeptides(CLP) i.e, CLP027A, CLP069A, Cepacidine A, CLP2011A and CLP02A were produced from each strains, respectively. Nitrogen and carbon sources in media were proved to be important factors for the production of CLP and among them, polypeptone-S, glucose and fructose were the most effective. It appeared that compounds CLP027A and CLP069A were identical with Cepacidine A and Xylocandine A, respectively. contain aspartic acid as amino acid component, are differentiated from Xylocandine A containing asparagine. Although molecular weight, amino acid composition and UV spectrum of CLP2011A and CLP02A are same with those of Cepacidine A, it is postulated that these compounds are not identical with Cepacidine A when the antifungal spectra and antifungal activity were compared to those of Cepacidine A.

토양으로부터 항진균 활성을 나타내는 5종류의 Pseudomonas cepacia 균주 AF027, AF069, AF2001, AF2011, SD02가 분리되었으며 이들은 각각 cyclic lipopeptide계 항진균 물질 CLP027A, CLP069A, Cepacidine A, CLP2011A, CLP02A를 생산하였다. 조사된 P cepacia 균주들은 배지중에 함유된 질소원 및 탄소원의 종류에 따라 항진균 물질 생산성에 현저한 차이가 있었는데, 질소원으로서는 polypeptone-S, 탄소원으로서는 glucose와 fructose가 생산성 증대에 가장 효과적이었다. 항진균물질 $CLP069A_1$과 $CLP069A_2$는 각각 분자량이 1215와 1199이며, 아미노산 조성, UV spectrum, IR spectrum 및 항진균 스펙트럼에 있어서, Xylocandin $A_1,\;A_2$와 일치하였으며, CLP027 $A_1$과 $A_2$ 또한 이러한 물리 화학적 특성이 Cepacidine A와 완전히 일치함으로써 동일 물질로 생각된다. $CLP2011A_1,\;A_2$와 $CLP02A_1,\;A_2$는 아미노산 조성 및 항진균 스펙트럼이 Xylocandin $A_1,\;A_2$와 다르고, Cepacidine $A_1,\;A_2$와는 항진균 스펙트럼에서 현저하게 차이가 났다. CLP2011A, CLP02A가 분자량, 아미노산 조성, UV spectrum, IR spectrum에서 Cepacidine A와 동일하지만 항진균 스펙트럼이 상이한 것은 이들이 다른 물질임을 시사하며, 이는 aliphatic side chain이 Cepacidine A의 그것과 다른 데에서 비롯된 분자구조의 상이함에서 기인된 것으로 추론된다.

Keywords

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