BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Immobilization and Characterization of a Liposome-Mediated Reconstituted Nicotinic Acetylcholine Receptor

  • Suh, Jeong-Ihn (Department of Biochemistry and Bioproducts Research Center, Yonsei University) ;
  • Palk, Bo-Hyun (Department of Biochemistry and Bioproducts Research Center, Yonsei University) ;
  • Oh, Se-Zu (Department of Chemistry, Seoul National University) ;
  • Suh, Jung-Hun (Department of Chemistry, Seoul National University) ;
  • Cho, Key-Seung (Department of Biochemistry, Hanyang University) ;
  • Palk, Young-Ki (Department of Biochemistry and Bioproducts Research Center, Yonsei University)
  • Received : 1994.10.19
  • Published : 1995.03.31
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Abstract

A nicotinic acetylcholine receptor (nAchR) isolated from the electric tissues of Torpedo californica has been reconstituted into a vesicle comprising a bifunctional azo-ligand (Bae 1) compound, and a liposome containing phospholipids and cholesterol (1 : 1, w/w). The liposome-mediated reconstituted receptor showed a concentration-dependent response to cholinergic drugs in a lithium ion flux assay. This liposome-mediated reconstituted nAchR was immobilized onto an electrode using various synthetic polymers which were tested for their response to the cholinergic ligands. The immobilized nAchR not only exhibited a linear response to a wide range of cholinergic ligand concentrations but also retained an operational stability which lasted for longer than 6 days. Thus, this result provides a basis for application of the immobilized nAchR-based biosensor in detecting cholinergic ligands in vitro.

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