Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
권호 표지

A METHOD OF MUCOSA CULTURE

구강점막의 배양에 관한 연구

  • Choi, Byung-Ho (Department of Oral Maxillofacial Surgery, College of Dentistry Yonsei University) ;
  • Yoo, Jae-Ha (Department of Oral Maxillofacial Surgery, College of Dentistry Yonsei University)
  • 최병호 (연세대학교 치과대학 구강악안면외과학교실(원주기독병원)) ;
  • 유재하 (연세대학교 치과대학 구강악안면외과학교실(원주기독병원))
  • Published : 1995.12.31
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Abstract

To use cultured mucosa as a graft of full thickness, our laboratory has been involved in the development of techniques to grow epidermis together with connective tissue. Human oral mucosa was obtained at dental surgery. Under sterile conditions the tissues were cut into explants of 0.1 $cm^2$ which were placed in the center of 24 well tissue culture dishes and incubated in a growth medium. The growth medium used for epithelial was MEM(Minimum Essential Medium) supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide, glutamine (0.292 g/l), epidermal growth factor (40 ug/ml), cholera toxin (30 ng/ml), hydrocortisone (2 ug/ml), insulin (40 ug/ml) and transferin (5 ug/ml). The medium for stratification of epithelial cells was MEM supplemented with 10% fetal calf serum, 0.5% dimethyl sulfoxide and glutamine (0.292 g/l). The medium used for fibroblasts was MEM supplemented with 10% fetal calf serum. With the three types of media used alternatively, a mucosa composed of epidermis and connective tissue was obtained after 3 weeks of culture.

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