Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Hypoglycemic Effect of Polygonatum Odoratum var. Pluriflorum Ohwi Extract in Streptozotocin-Induced Diabetic Rats

둥굴레(Polygonatum Odoratum var. Pluriflorum Ohwi)추출물의 당뇨 유발 흰쥐에 대한 혈당강화 효과

  • 임숙자 (덕성여자대학교 자연과학대학 식품영양학과)
  • Published : 1995.09.01
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Abstract

The hypoglycemic effect of Polygonatum odoratum var. Pluriflorum Ohwi was investigated after extracted with methanol. The methanol extract was fractionated into 4 layers ; hexane, chloroform, butanol and aqueous. Fifty male Sprague-Dawley rats(200-300g) were induced diabetes mellitus by the streptozotocin injection(45mg/kg B.W) into the tail vein and were divided into 5 groups ; diabetic control and 4 experimental groups. All groups of the rats were fed on a AIN-76 diet, and the 4 experimental groups were orally administered with each fraction(500mg/kg B.W) for 12 days and the diabetic control group was orally administered CMC. The body weight gain was monitered and the blood levels of glucose and cholesterol were measured. Levels of protein, triglyceride, and free fatty acid in plasma were analysed. The contents of protein, triglyceride and glycogen in liver and of protein and glycogen in muscle were also determined. The body weight gain was shown significantly higher in the H2O fraction group than that in the diabetic control group and the heart weight was greater in the CHCl3 group. The extents of blood glucose decrement were greater in the BuOH and H2O groups than that in the control group. The urinary glucose excretion was shown relatively small amount in the BuOH and H2O groups. The plasma cholesterol and protein levels were not influenced by these four fractions in diabetic rats. The liver glycogen level was significantly higher in the BuOH group. The results suggest that the orally administered BuOH and H2O fraction of Polygonatum odoratum var. Pluriflorum Ohwi exhibited hypoglycemic effect in streptozotocin induced diabetic rats.

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References

  1. Diabetic Care v.12 Traditional plant medicines as treatments for diabetes Bailey,C.J.;Day,C.
  2. Planta Medica v.50 no.2 Effects of Euphobia prostrata and Fumaria parvflora in normoglycaemic and alloxan-treated hyperglycemic rabbits Akhtar,M.S.;Kham,Q.M.;Khaliq,T.
  3. Planta Medica v.52 no.1 Tormentic acid, a new hypoglycemic agent from Poterium ancistroides Villar,A.;Paya,H.;Hortiguela,M.D.;Cortes,D.
  4. Planta Medica v.55 no.4 Hypoglycemic and insulin release effects of tormentic acid : a new hypoglycemic natural product Iyorra,M.D.;Paya,H.
  5. Planta Medica v.56 Hypoglycemic effect of Momordica charantia extracts Day,C.;Cartwright,T.;Provost,J.;Bailey,C.J.
  6. Planta Medica v.57 Hypoglycemic effect of swerchirin from the hexane fraction of Swertia chirayita Bajpai,M.B.;Asthana,R.K.;Sharma,N.K.;Chatterjee,S.K.;Mukherjee,S.K.
  7. Planta Medica v.51 Study of hypoglycemic activity of Cuminum nigrum seeds in normal and alloxan diabetic rabbits Akhtar,M.S.;Ali,M.R.
  8. J Kor Agric Chem Soc v.31 no.1 산채류 가열즙의 돌연변이 억제작용에 관한 연구 함승시
  9. Kor J Pharmacogn v.21 no.2 A preliminary study on hypocholesterolemic and hypoglycemic activities of some medicinal plants Choi,J.S.;Chung,H.Y.;Han,S.Y.
  10. Thesis collection. SM Pharmacogn Sci v.7 Studies on the physiochemical activities of Commelinae Herba extract on the normal and the streptozotocin-in-duced hyperglycemic rats Kim,T.H.;Yang,K.S.;Whang,S.H.
  11. 석사학위논문, 덕성여자대학교 Hypoglycemic effects of Commelina communis L. and characterization of glucose-6-phosphate dehydrogenase in rat liver 박수영
  12. J Korean soc Food Nutr v.17 no.1 Effects of Aralia elata extract on experimentally alloxan induced diabetes in rabbits Lee,M.Y.;Lee,J.S.;Sheo,H.J.
  13. 박사학위논문, 서울여자대학교 두릅나무 근피 추출물의 혈당강하 효과 김옥경
  14. 성대논문집 v.31 Pharmacognostic studies on Polygonatum sibricum Redoute Lee,Y.C.;Won,D.H.
  15. J Nutr v.107 Report of the American Institute of Nutrition;Ad Hoc Committee on Standards for nutritional studies American Institute of Nutrition
  16. J Nutr v.110 Second report of the Ad Hoc Committee on Standards for nutritional studies American Institute of Nutrition
  17. Diabetes v.21 no.3 Streptozotocin-induced diabetes and islet cell alterations in rabbits Lazarus,S.S.;Shapiro,S.H.
  18. J Clin Invest v.48 Diabetogenic action of streptozotocin : relationship of dose to metabolic response Alain,J.;Andre,E.;Werner,S.;Albert,E.R.
  19. Scand J Clin & Lab Invest v.12 On the enzymatic determination of blood glucose Raabs,E.;Terkildsen,T.C.
  20. J Biol Chem v.177 Determination of serum protein by means of the biuret reation Gomall,A.G.;Bardawill,C.S.;David,M.M.
  21. 탐구실험생화학 한국 실험 생화학회 편찬위원회
  22. J Clin Chem v.21 Serum triglyceride determinated colorimetry with on enzyme that produces hydrogen peroxide Giegel,J.L.;Ham,A.B.;Clema,W.
  23. Clin Chim Acta v.466 An easy colorimetric micromethod for routine determination of free fatty acid in plasma Falholt,K.;Lund,B.;Falholt,W.
  24. Methods in Enzymology 3 Chemical procedures for analysis of polysaccharides Hassid,W.Z.;Ahraham,X.
  25. Statistical Analysis System SAS Institute Inc.
  26. Acta Endocrinol(Copehn) v.114 Diabetogenic action of streptozotocin : essential role of membrane permiability Beppu,H.;Maruta,K.;Kurner,T.;Kolb,H.
  27. Diabetes v.35 Streptozotocin diabetes alters immunoreactive β-endorphin levels and pain perception after 8wk in female rats Forman,S.;Estilow,M.L.;Vailenko,P.
  28. Endocrinology v.119 no.6 Phenylethanolamin N-methyl transferase in the basis of streptozotocin diabetic rats Fisher,K.J.;Stewart,J.K.
  29. Human Physiology(5th ed.) Vander,A.J.;Sherman,J.H.;Luciano,D.S.
  30. Metabolism v.22 Plasma triglyceride transport kinetics in diabetes mellitus Nikkila,E.A.;Kekki,M.
  31. J Biol Chem v.186 Lipogenesis and glucose oxidation in the liver of the alloxan diabetic rat Chernick,S.S.;Chaikoff,I.L.;Masoro,E.J.;Isaeff,E.
  32. Diabetes v.39 Cholesterol metabolism in alloxan-induced diabetic rabbits O'Meara,N.M.G.;Devery,R.A.M.;Owens,D.;Collins,P.B.;Johnson,A.H.;Tomkin,G.H.
  33. J Nutr v.117 Acute alloxan diabetes alters the activity but not the total quantity of acetyl-CoA carboxylase in rat liver Roman-Ropez,C.R.;Allred,J.B.
  34. Eur J Biochem v.146 Decrease of fatty acid oxidation, ketogenesis and gluconeogenesis in isolated perfused rat liver by phenylalkyl oxirane carboxylate due to inhibition of phenyloxirane carboxylate due to inhibition of CPT1. Wolf,H.O.P.;Engel,D.W.
  35. Diabetes v.39 Insulino-mimetic properties of trace elements and characterization of their in vivo mode of action Rosseti,L.;Giaccari,A.;Klein-Robbenhaar,E.;Vogel,L.R.
  36. 전통 약물로 부터 신약 개발 연구법 당뇨병 치료제의 검색 방법 정성현
  37. Diabetes v.34 Renal hypertrophy in experimental diabetes : changes in pentose phosphate pathway activity Steer,K.A.;Sochor,M.;Mclean,P.