Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Phytate on the Protein digestibility and Availability in vitro of Calcium, Iron and Zinc in Soymilk Treated with Phytase

피타아제를 처리한 두유의 단백질 소화율과 칼슘, 철, 아연의 유동도에 대한 피트산의 효과

  • 황인경 (서울대학교 가정대학 식품영양학과)
  • Published : 1995.11.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was to examine the effect of phytate on the protein digestibility and calcium, iron and zinc availability in phytase treated soymilks digested with pepsin and pepsin-pancreatin in vitro. Also, the bending between phytate and protein in soymilks was investigated by means of SDS-PAGE. The content of phytate in soymilk was reduced by phytase treatment. As the content of phytate decreased, the protein digestibility increased in soymilk treated with the digest enzymes in vitro. The reduction of phytate content in soymilk improved the availability of all calcium, iron and zinc. Although the availability of calcium increased, the amount of change was small. The phytate reduction increased most the availability of iron. A number of bands of high molecular weight protein in soymilk disappleared in SDS-PAGE by lowering the phytate content with phytase treatement on soymilk.

Keywords

References

  1. J Nutr v.90 Dietary metal complexing agents and zinc availability in the rat Oberleas D.;Muhrer M.E.;O'Dell B.
  2. J Nutr v.110 Metabolism of ${{^{14}C-$phy-tate in rats : Effect of low and high dietary calcium intakes Nahapetian A.;Young V.R.
  3. J Food Sci v.47 Phytic acid hydrolysis and soluble zinc and iron in whole wheat bread as affected by calcium containing additives Zemel M.B.;Shelef L.A.
  4. Proc Nutr Soc v.26 A critical evaluation of the mineral and nitrogen balance in man Isaksson B.;Sjogren G.
  5. Proc Soc Exp Biol Med v.159 Body iron losses in animals Finch C.A.;Ragan H.A.;Dyer I.A.;Cook J.D.
  6. Am j Clin Nutr v.29 Measurement of iron absorption from composite meals Bjorn Rasmussen E.;Hallberg L.;Magnusson B.;Rossander L.;Svanberg G.;Arvidsson B.
  7. J Food Sci v.54 Effects of pH on calcium, zinc and phytate solubilities and complexes following in vitro digestions of soy protein isolate Champagne E.T.;Phillippy B.Q.
  8. Am J Clin Nutr v.34 An in vitro method for estimation of iron availability from meals Miller D.D.;Schricker B.R.;Rasmussen R.R.;Van Campen D.
  9. J Agric Food Chem v.40 Digestibility of technologically treated lamb meat samples evaluated by an in vitro multienzymatic method Restani P.;Restelli A.R.;Capuano A.;Galli C.L.
  10. Korean Food industry v.52 Development and view of the technology preparing soymilk Lee C.H.
  11. J Agric Food Chem v.28 A simple and rapid colorimetric method for phytate determination Latta M.;Eskin M.
  12. J Food Sci v.50 Effect of phytate and partially hydrolyzed phytate on in vitro protein digestibility Knuckles B.E.;Kuzmicky D.D.;Betschart A.A.
  13. J Biol Chem v.193 Protein measurement with the folin phenol reagent Lowry O.H.;Rosebrough N.J.;Farr A.L.;Randall R.J.
  14. A.O.A.C. : Offical Methods of Analysis(15th ed.)
  15. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli U.K.
  16. J Sci Food Agric v.40 Effects of phytate on mineral bioavailability. In vitro studies on $Mg^ {2+}, Ca^ {2+}, Fe^ {2+},Cu^ {2+} and Zn^ {2+}(also Cd^ {2+})$ solubilities in the presence of phytate Nolan K.B.;Duffin P.A.;McWeeny D.J.
  17. Cereal Chem v.63 Nutrient, antinutrient contents, and solubility profiles of nitrogen, phytic acid, and selected minerals in winged beans flour Kantha S.S.;Hettiarachy N.S.;Erdman Jr.J.W.
  18. Am J Clin Nutr v.31 An in vitro method for predicting the bioavailability of iron from foods Rao B.S.N.;Prabhvathi T.
  19. J Food Sci v.49 In vitro evaluation of the effects of ortho-, tripoly- and hexametaphosphate on zinc, iron and calcium bioavailability Zemel M.B.
  20. J Agric Food Chem v.35 Electrophretic analysis of whey proteins present in soybean globulin fractions Iwabuchi S.Yamauch F.
  21. Cereal Chem v.64 High resolution sodium dodesyl sulfate polyacrylamide gel electrophoresis of soybean(Glycine max L.) seed proteins Sathe S.K.;Lilley G.G.;Mason A.C.;Weaver C.M.
  22. Cereal Chem v.63 Quantitative analysis of soybean proteins by densitometry on gel electrophoresis Sato K.;Yamagishi T.;Yamauchi F.
  23. J Agric Food Chem v.33 Effect of phytate removal treatments upon the molecular weight and subunit composition of major soy protein fractions Brooks J.R.;Morr C.V.
  24. J Agric Food Chem v.39 Phytate-mineral-protein composition of soybeans : Gel filtration studies of soybean meal extracts Honing D.H.;Wolf W.J.