Effect of Phosphatase Activity on Product of $\beta$-galactosidase by E. coli P90C

Phosphatase 의 활성이 E. coli P90C의 $\beta$-galactosidase생성에 미치는 영향

ATPase was the most available phosphatase in culture broth of 5. coli P90C. To measure the stable phosphatase activity it was necessary to react nth reaction reagent over 30 min and then we can get stable optical density at 410 m. Transfer time from preculture to main culture for the production of $\beta$-glactosidase was good after 3 hrs cultivation. Phosphatase activity was highest at log phase in main culture and as the cell begins to make $\beta$-galactosidase phosphatase activity begins to decrease.