Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Dextranase Produced by Flavobacterium multivorum HL-1

Flavobacterium multivorum HL-1 이 생산하는 Dextran 분해효소의 특성

  • 서은숙 (건국대학교 이과대학 생물학과) ;
  • 유관희 (상지대학교 이과대학 생물학과) ;
  • 이형환 (건국대학교 이과대학 생물학과)
  • Published : 1995.02.01
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Abstract

Dextranase produced by Flavobacterium multivorum HL-1 isolated from soil were characterized. Optimum growth condition for the production of the enzyme by the strain appeared to be at the 1.0% concentration of dextran. When NH$_{4}$N0$_{3}$ and beef extract was added to the culture media, the enzyme activity increased upto 570 and 680 units per ml respectively, and other nitrogen sources did not increase the activity. Urea, casamino acid, (NH$_{4}$)$_{2}$SO$_{4}$ and KNO$_{3}$ inhibited the activity. In the presence of Mg^{2+} in culture media, the enzyme activity increased 124%, but its activity was inhibited in the presences of Ca$^{2+}$, Co2$^{2+}$, Hg^{2+} and Zn$^{2+}$ . The optimum temperature for the enzyme activity was 45-55$\CIRC$C . In the ranges of pH 4 to 10, the activity of the enzyme appeared approximately similar.

Keywords

References

  1. Carbohyd. Res v.22 Studies on dextrans and dextranases. Part X. Types and percentages of secondary linkages in the dextrans elaborated by Luconostoc mesenteroides NRRL B-1299 Bourne,E.J.;R.L.Sidebotham;H.Weigel
  2. Carbohyd. Res v.31 The structure of a dextran produced by Leuconostoc mesenteroides NRRL B-1397: The linkages and length of the branches Miyaji,H.;A.Misaki
  3. J. Bacteriol v.98 Dextraninduced agglutination of Streptococcus mutans and its potential role in the formation of microbial dental plaques Gibbons,R.J.;R.J.Fitzgerald
  4. Kor. J. Microbiol v.18 Characterization of Streptococcus mutans isolated from human dental plaque. 2. Streptococcal polysaccharide Lee,K.J.;B.H.Lee;
  5. Appl. Microbiol v.24 Distribution of enzyme forming polysaccharides from sucrose and the composition of extracellular polysaccharide by Streptococcus mutans Robrish,S.A.;W.Reid;M.Krichevsky
  6. Science v.115 Enzymatic hydrolysis of dextran Whiteside-Carlson;V.;W.W.Carlson
  7. J. Bacteriol v.64 Dextran-degrading enzymes from molds Tsuchiya,H.M.;A.Jeans;H.M.Bricker;C.A.Wilham
  8. Biochem. J v.88 Studies on dextrans and dextranases. IV. Mechanisms of the actions of intra-and extra-cellular mould dextranases Hutson,D.H.;H.Weigel
  9. J. Biochem v.71 Studies on mold dextranases. Ⅲ. Purification and some enzymatic propertis of Aspergillus carneus dextranase Hiraoka,N.;J.Fukumoto;D.Tsuru
  10. Kor. J. Microbiol v.18 Studies on mold dextranases. 1. Dextranase production by a strain of Aspergillus ustus Lee,K.J.
  11. Carbohyd. Res v.6 Cell-bound exodextranase of Bacillus species Zevenhuizen,L.P.T.M
  12. Agric. Biol. Chem v.47 no.11 An isomaltotriose producing dextranase from Flavobacterium sp. M-73; Purification and properties Kobayashi,M.;S.Takaki;M.Shiota;Y.Mitsuishi;K.Matsuda
  13. Agric. Biol. Chem v.43 no.11 Dextran α-1,2 debranching enzyme from Flavobacterium sp. M-73∴ Its production Mitsuishi,Y.;M.Kobayashi;K.Matsuda
  14. Carbohyd. Res v.83 Dextran α-(1-2) debranching enzyme from Flavobacterium sp. M-73. Properties and mode of action Mitsuishi,Y.;M.Kobayashi;K.Matsuda
  15. Carbohyd. Res v.54 Purification and substrate specificity of an endo-dextranase of Streptococcus mutans K1-R Pulkownik,A.;G.J.Walker
  16. J. Gen. Microbiol v.127 Metabolism of the polysaccharides of human dental plaque; release of dextranase in batch cultures of Streptococcus mutans Walker,G.J.;A.Pulkownik;J.G.Morrey Jones
  17. Carbohyd. Res v.38 The structure of water-insoluble glucans of cariogenic Streptococcus mutans, formed in the absence and presence of dextranase Ebisu,S.;A.Misaki
  18. Bull. Tokyo. Med. Dent. Univ v.23 Dextran degrading bacteria in human oral cavity and their activity against insoluble glucan from Streptococcus mutans Matsuda,Y.
  19. J. Dent. Res. Special Issue C Immunologic basis for vaccination against dental caries in Rhesus Monkeys Lehner,T.;S.J.Challacombe;J.Caldwell
  20. Manual of the identification of medical bacteria(2nd ed.) Cowan,S.T.;K.J.Steel
  21. J. Biol. Chem v.160 A new reagent for the determination of sugars Somogyi,M
  22. Methods in Enzymolog v.8 A Bacterial dextranase Janson,J.C.;J.Porach
  23. Bichem. Biophys. Res. Commu v.80 Pronounced hydrolysis of highly branded dextrans with a new type of dextranase Kobayashi,M.;Y.Mitsuishi;K.Matsuda
  24. Biochemica et Biophysica Acta v.309 Studies on dextranase. Purification of dextranase from Penicillium funiculosum and its enzymatic properties Sugiura,M.;A.Ito;T.Ogiso;K.Kato;H.Asano
  25. Biochemica et Biophysica Acta v.350 Studies on dextranase Ⅱ. New exo-dextranase from Brevibacterium fuscum var. dextranlyticum Sugiura,M.;A.Ito;F.Yamaguchi
  26. J. Biochem v.69 Studies on mold dextranases. I. Penicillium luteum dextranase: Its production and some enzymatic properties Fukumoto,J.;H.Tsuji;D.Tsuru
  27. Agr. Biol. Chem v.37 no.11 Production and properties of alkaline dextranase from a newly isolated Brevibacterium Yamaguchi,T.;S.Gocho
  28. A bacteral dextranase. Biochem. J v.72 Bailey,R.W.;R.T.J.Clarke