Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of a Cell Aggregation Factor from Aspergillus sp.LAM 94-142

Aspergillus sp. LAM 94-142가 생산하는 세포응집물질의 특성

  • 이동희 (건국대학교 미생물공학과) ;
  • 함동수 (건국대학교 미생물공학과)
  • Published : 1995.10.01
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Abstract

A cell aggregation factor produced by Aspergillus sp. LAM 94-142 was purified and partially characterized. The factor was purified about 15 folds from culture broth by IRA 420 and IRC 120 treatment, 1% NaCl added acetone precipitation, and Sepharose 4B column chromatography with overall yield of 48%. It was heteropolysaccharide consisted of mannose, arabinose, and glucose with a molar ratio, 31:17:2, and its molecular weight was estimated to be about 900,000 daltons by Sepharodse 4B gel filtration method. The optimum pH and temperature was 8 and 40$\circ$C, respectively. The factor was stable in pH range of 3-9 and at 100$\circ$C for 90 min. The cell aggregation activity of the factor was inhibited by the addition of Hg$^{2+}$, Fe$^{2+}$, Cu$^{2+}$, and some polypeptides such as milk casein or hemoglobin. The factor aggregated Bacillus subtilis, B. macerans, B. turingiensis, E. coli, Peudomonas aeruginosa, P. fluorescens, P. malophilia, and weakly aggregated Staphylococcus sp., Sarcina lutea, P. putida and Cryptococcus neoformnans, but it didn't aggregate various strains of Candida sp. and Saccharomyces sp.

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References

  1. Science v.177 Lectins: cell-aggutination and sugra-specifid proteins Sharon,N.;H.Lis
  2. Ann. Rev. Biochem. v.42 The biochemistry of plant lectins Lis,H.;N.Sharon
  3. Agric. Biol. Chem. v.46 Purification of a new L-fucose-specific letin produced by Streptomyces No.100-2 Matsui,I.;T.Kameyama;K.Oishi;K.Aida
  4. J. Biochem. v.97 The morphology of L-fucose specific lectin(SFL 100-2)produced by Streptomyces No. 100-2 Matsui,I.;K.Oishi;K.Kanaya;N.Baba
  5. 생화학뉴스 v.5 렉틴 정시련;정경희
  6. Agric. Biol. Chem. v.52 Cell aggregation factor produced by Streptomyces sp. strain No. A-3315 Suzuki,K.;N.Nakano;R.Tanaka;M.Uyeda;M.Shibata
  7. Agric. Biol. Chem. v.54 HAF hepatoma aggregation factor produced by Streptomyces sp. strain No, A-6143 Suzuki,K.;N.Nakano;Y.Nagatomi;H.Tominaga;N.Nakazono;M.Itai;M.Uyeda;M.Shibata
  8. Agric. Biol. Chem. v.51 SAF, a new cell aggtegation factor produced by Streptomyces murinus strain No. A-2805 Suzuki,K.;M.Ksai;K.Yolomizo;M.Uyeda;M.Shibata
  9. J. Antibiotics v.33 Canacelin, a cancer cell agglutinin from Streptomyces sp. Ikekawa,T.;T.Asari;T.Manabe;M.Umeji;S.Yanoma
  10. Agric. Biol. Chem. v.52 Characteristics and action mechanism of SAF, cleeaggergation factor produced by Streptomyces murinus strain No. A-2805 Suzuki,K.;N.Nakano;R.Tanaka;M.Uyeda;M.Shibata
  11. Agric. Biol. Chem. v.54 3315 Af2, a cell aggregation factor produced by Streptpmyces sp. strain No. A-3315 Uyeda,M.;K.Suzuki;M.Shibata
  12. Agric. Biol. Chem. v.40 Screengin, isolation and some properties fo microbial cell flocculants Nakamura,J.;S.Miyashiro;Y.Hirose
  13. Agric. Biol. Chem. v.40 Purification and chemical analysis of microbial cell flocculant produced by Aspergillus sojae AJ 7002 Nakamura,J.;S.Miyashiro;Y.Hirose
  14. Agric. Biol. Chem. v.40 Modes of flocculation of yeast cells with flcculant produced by Aspergillus sojae AJ7002 Nakamura,J.;S.Miyashiro;Y.Hirosd
  15. Agric. Biol. Chem. v.49 Flocculant production by Paecilomyces sp.taxonomic studies and culture conditions for production Takagi,H.;K.Kadowaki
  16. Agric. Biol. Chem. v.49 Purification and chemical properties of a flocculant produced by Paecilomyces Takagi,H.;K.Kadowaki
  17. Agric. Biol. Chem. v.50 Screening for and characteristics of microbial flocculants Kurane,R.;K.Takeda;T.Suzuki
  18. Agric. Biol. Chem. v.50 Culture conditions for production of microbial flocculant by Rhodococcus erythropolis Kurane,R.;K.Takeda;T.Suzuki
  19. 생물화공 v.4 Zoogloea ramigera에 의한 biopolymer생산과 환경분야 이용 정윤철
  20. 생물화공 v.4 미생물 polysaccharide의 생합성과 물성 이신영
  21. 한국생물공학회지 v.7 Zoogloea ramigera에 의한 생물고분자 생산에 관한 연구 안대희;권해수;정윤철
  22. Agric. Biol. Chem. v.55 Microbial flocculant from Alcaligenes cupidus KT201 Toeda,K.;R.Kurane
  23. Agric. Biol. Chem. v.55 Microbial flocculation of waste liquids and oil emulsion by bioflocculant from Alcalogenes latus Kurame,R.;Y.Nohata
  24. J. Gen. Appl. Microbiol. v.25 Sugar specificity of the hemagglutinins produced by soil bacteria Takenka,A.;K.Oishi;K.Aida
  25. Biosci. Biotech. Biochem. v.56 Effect of proteases on hepatoma AH109A cells aggregated by a microbial hipatoma aggregation(HAF) Suzuki,K.;H.Tominaga;M.Uyeda
  26. Agric. Biol. Chem. v.39 screening, isolation and some properties of mouse tumor cell agglutinin Oishi,K.;K.Aida