Journal of Microbiology

  • 제33권4호
  • /
  • Pages.289-294
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  • 1995
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Physiological characterization of kinetics and action mechanism of vibrio hemolysin

  • Choe, Young-Chool (Research Center for Molecular Microbiology, Seoul National University) ;
  • Jeong, Cajin (Research Center for Molecular Microbiology, Seoul National University)
  • 발행 : 1995.12.01
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초록

The action mechanism of hemolysin rendering virulency of Vibrio anguilarum has not clarified as yet, even though there were several possible factors explained. We have studied hemolytic kinetics performed by hemolysin from V. anguillarum strain V7 as well as binding of hemolysin to RBC membrane. Maximal rate of hemolysis and duration of lag phase were directly and inversly correlated to the concentration of hemolysin used. Hemolysin molecules are known to bind consumptively with proper diameter, while other protectants with smaller diameter could not. In conclusion, hemolysin should bind irreversibly to RBC membrane exert hemolysis distorting osmotic pressure. The binding could be hindered by spatial structure of the RBC surfacem which might be caused by sialic acid.

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참고문헌

  1. J. Bacteriol. v.167 Characterization of anguibactin, a novel siderophore from Vibrio anguillarum 775(pJM1) Actis,L.A.;W.Fish;J.H.Crosa;K.Kellerman;S.R.Ellenberger;F.M.Hauser;J.Sanders-Loehr
  2. J. Biol. Chem. v.263 Genetic and molecular characterization of essential components of the Vibrio anguillarum plasmid mediated iron-transport system Actis,L.A.;M.E.Tolmasky;D.H.Farrel;J.H.Crosa
  3. Infect. Immun. v.31 Study of phospholipase C (heat-labile hemolysin) in Pseudomonas aeruginosa Berka,R.M.;G.L.Gray;M.L.Vasil
  4. J. Bacteriol. v.152 Escherichia coli hemolysin may damage target cell membranes by generating transmembrane pores Bhakdi,S.;N.Mackman;J.M.Micaud;I.B.Holland
  5. Infect. Immun. v.58 Calcium is required for binding of Escherichia coli hemolysin (Hly A) to erythrocytes membranes Boehm,D.F.;R.A.Welch;I.S.Snyder
  6. Ann. Rev. Microbiol. v.39 Mechanism of bacterial virulence Brubaker,R.P.
  7. J. Med. Microbiol. v.15 Cytotoxic activity of partially purified Escherichia coli alpha-hemolysin Cavalieri,S.J.;I.S.Snyder
  8. Kor. Jour. Microbiol. v.32 Virulence evaluation of Vibrio anguiliarum in accordance with hemolytic activity on Human Red Blood Cells Choe,Y.;G.Jeong
  9. Infect. Immun. v.31 Outer membrane proteins induced under conditions of iron limitation in the marine fish pathogen Vibrio anguillarum Crosa,J.H.;L.L.Hodges
  10. Infect. Immun. v.18 Evidence for plasmid contribution to the virulence of the fish pathogen Vibrio anguillarum Crosa,J.H.;M.H.Schiewe;S.Falcow
  11. Microbial. Rev. v.47 Membrane receptors for bacterial toxins Eidels,K.;R.L.Proia;D.A.Hart
  12. J. Fish Dis. v.6 The role of extracellular products of Vibrio anguillarum Kanemori,Y.;K.Muroga;T.Nakai
  13. Fish Pathol. v.20 Partial purification of extracellular substance of Vibrio anguillarum toxigenic for rainbow trout and mouse Kodama,H.;M.Mustafa;T.Mikami;H.Izawa
  14. Kor. Jour. Microbiol. v.32 Characterization of the nature of virulency caused by Vibrio anguillarum: Identification of an outer membrane protein rendering virulency Lee,J.S.;G.Jeong;Y.C.Hah
  15. Production and properties of a hemolytic toxin by Vibrio anguillarum In Fish disease, Third COPRAQ session Munn,C.B.;Ahne,W.(ed.)
  16. J. Med. Microbiol. v.7 The kinetics of erythrocyte lysis by Escherichia coli hemolysin Renine,R.P.;J.H.Freer;J.P.Arbuthnott
  17. J. Bacteriol. v.9 Properties of the hemolytic activity of Escherichia coli Short,E.C.Jr.;H.J.Kurtz
  18. J. Gen. Microbiol. v.24 A disease of finnock due to Vibrio anguillarum Smith,I.W.
  19. J. Bacteriol. v.9 Production and characterization of hemolysis of Escherichia coli Snyder,I.S.;N.A.Koch