Journal of Microbiology

  • 제33권4호
  • /
  • Pages.283-288
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  • 1995
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Identification of hemolysin as one of the important virulent factors in vibrio anguillarum V7

  • Choe, Young-Chool (Research Center for Molecular Microbiology, Seoul National University) ;
  • Jeong, Gajin (Research Center for Molecular Microbiology, Seoul National University)
  • 발행 : 1995.12.01
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초록

We have identified hemolysin rendering virulency of Vibrio anguilarum grown at 23.deg.C which was evaluated on human RBCs. Hemolysin itself was separated as a single band on non-denaturing gel electrophoresis. Vibrio hemolysin was destroyed by trypsin and proteinase K and was heat labile. Optimal pH for activity was around pH 6 while pI of the molecule was recognized as 5.7, with relative distance (R$\sub$f/) on non-denaturing gel was 0.7. Addition of EDTA and FeCI$\sub$3/ drew the possibility that the production of hemolysin was mainly induced to overcome iron deficiency inside host animalsd infection.

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