Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Interstrain polymorphisms of isoenzyme profiles and mitochondrial DNA fingerprints among seven strains assigned to Acanthamoeba polyphaga

대식가시아메바(Acmthamoebapokphaga) 일곱 분리주간의 동위효소 profile과 Mitochondria DNA fingerprint의 다양성

  • Gong, Hyeon-Hui (Department of Parasitology, Kyungpook National University School of Medicine) ;
  • Park, Jun-Hyeong (Department of Parasitology, Kyungpook National University School of Medicine) ;
  • Jeong, Dong-Il (Department of Parasitology, Kyungpook National University School of Medicine)
  • 공현희 (경북대학교 의과대학 기생충학교실) ;
  • 박준형 (경북대학교 의과대학 기생충학교실) ;
  • 정동일 (경북대학교 의과대학 기생충학교실)
  • Published : 1995.12.01
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Abstract

Interstrain polymorphisms of isoenzyme profiles and mitochondrial (Mt) DNA fingerprints were observed among seven strains of Acnnthnmoeba isolated from different sources and morphologically assigned to A. polvphngn. Mt DNA ringerprints by eight restriction endonucleases (Bgl II, Sca I, Cla I, EcoR I, Xbo I, Kpn I, Sal I, and Sst I) revealed considerable interstrain polymorphisms . Isoenzyme profiles revealed considerable interstrain polymorphisms for acid phosphatase, lactate dehydrogenase, and glucose-6- phosphate dehydrogenase while those for glucose phosphate isomerase , leucine aminopeptidase , and malate dehydrogenase showed similarity Despite of the interstrain polymorphisms, the isoengyme profiles and Mt DNA fingerprints of the strain Ap were found to be identical with those of the strain .tones . Mt DNA fingerprinting was found to be highly applicable for the strain identification, characterization, and differentiation. Key words: Acanthnmoebn polyphcga, interstrain polymorphism, isoenzyme profiles , Mt DNA fingerprints, strain differentiation, strain identification.

형태적으로 Aconthamoeba polyphaga로 동정긴 일곱 분리주의 동위효소 profile과 Mitochondria (Mt) DNAangerprint를 비교 분석하였다. 8가지 제한효소(B91 II. Sca I, Cla I, EcoR I, Xba I, Kpa I, Sal I. 및 Sst I)에 의한 Mt DNA fhlgerprint는 주간의 심한 다양성을 나타내었다. B가지 동위효소 (acid phosphatase, lactate dehydrogenase 및 glucose-6-phosphate dehydrogenase)는 주간의 심한 다양성을 나타내었으나 다른 3가지 동위효소(glucose phosphate isomerase, leucine aminopeptidase 및 malatedehydrogenase)는 비슷한 양상으로 나타났다 Ap 주의 동위효소 양상과 Mt DNA fmgerprint는 Jones 주와 동일하였다. Mt DNA fingerprinting은 대식가시아메바 주의 동정과 분리에 매우 유용함을 알았다. Aconthamoeba polvphasa의 우리말 학명을 대식가시아메바로 제안한다.

Keywords