• 한국미생물·생명공학회지
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• 제22권2호
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• pp.164-168
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• 1994

Identification of the Actinomycetes isolate strain No. 1372, a producer of Actinomycin X$_{2}$ was performed by using ISP method.l The strain, designated as No. 1372, was identified as Streptomyces floridae based on its morphological, physiological and biochemical characteristics. The highest production of the antibiotics by the strain was achieved in a fermentation medium containing soluble starch, yeast extract, (NH$_{4}$)SO$_{4}$, K$_{2}$HPO$_{4}$, NaCl$_{2}$, CaCO$_{3}$, and trace element.

• Structural Engineering and Mechanics
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• 제27권4호
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• pp.409-424
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• 2007

In this paper, an identification method of impact force is proposed for composite structures. In this method, the relation between force histories and strain responses is first formulated. The transfer matrix, which relates the strain responses of sensors and impact force information, is constructed from the finite element method (FEM). Based on this relation, an optimization model to minimize the difference between the measured strain responses and numerically evaluated strain responses is built up to obtain the impact force history. The identification of force history is performed by a modified least-squares method that imposes the penalty on the first-order derivative of the force history. Moreover, from the relation of strain responses and force history, an error vector indicating the force location is defined and used for the force location identification. The above theory has also been extended into the cases when using acceleration information instead of strain information. The validity of the present method has been verified through two experimental examples. The obtained results demonstrate that the present approach works very well, even when the internal damages in composites happen due to impact events. Moreover, this method can be used for the real-time health monitoring of composite structures.

• Entomological Research
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• 제48권5호
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• pp.339-347
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• 2018

Seven different strains of Beauveria bassiana were used in a bioassay on Pieris rapae larvae. The results showed that an B. bassiana strain showed relatively high pathogenicity towards P. rapae larvae. The adjusted mortality rate was 92.86 %, and the infection rate was 85.71 % in 10 days post inoculation. Molecular identification was performed to identify the unknown strain. Internal Transcribed Spacer sequence analysis showed that the polymerase chain reaction amplicon length of the unknown strain of Beauveria sp. was 573 bp, and sequence similarity to the known B. bassiana sequences in the NCBI database was 99 %. The B. bassiana strain was named Bb01. The changes of proteins and PPO of P. rapae larvae infected by B. bassiana Bb01 strain at different times was determined. The activity of PPO increased in 1-6 d and decreased in 7 d again after inoculation. The B. bassiana invaded into the insect body affected the balance of the proteins and PPO.

• Journal of applied mathematics & informatics
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• 제23권1_2호
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• pp.461-470
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• 2007

Parameter identification is studied in viscoelastic rods by solving an inverse problem numerically. The material properties of the rod, which appear in the constitutive relations, are recovered by optimizing an objective function constructed from reference strain data. The resulting inverse algorithm consists of an optimization algorithm coupled with a corresponding direct algorithm that computes the strain fields given a set of material properties. Numerical results are presented for two model inverse problems; (i)the effect of noise in the reference strain fields (ii) the effect of minimal reference data in space and/or time data.

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.205-209
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• 2003

Recently, we introduced a new method for rapid screening of bacterial species- or subspecies-specific DNA probes, named “inverted dot blot hybridization screening method”. We then applied this method to develop species- or strain- specific DNA probes for Prevotella intermedia and Prevotella nigrescens. In those studies, among 96 candidate DNA probes which were screened by the new method, 5 probes were confirmed as being putatively strain-specific : 3 probes for P. nigrescens 9336 (ATCC 33563), one for each p. intermedia ATCC 25611 and one for P. nigrescens G8-9K-3 (ATCC 49046). In the present study, we evaluated by Southern blot analysis a DNA probe Pi29-L, one of the 96 candidate probes described above, whether it is specific for the strain ATCC 25611 off. intermedia. Our data show that the probe Pi29-L is potentially P. intermedia ATCC 25611-specific, which can be useful for the detection and identification of the strain, particularly in maintenance of the strain.

• 한국미생물·생명공학회지
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• 제28권2호
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• pp.124-127
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• 2000

Beneficial bacteria, which have been used for medical purpose and for medicines for treating intestinal disorders, include strains of Bifidobacterium sp., Lactobacillus sp., Enterococcus sp., Clostridium butyricum, Lactobacillus sporogenes, Bacillus subtilis, Bacillus polyfermenticus and the like. Bacillus polyfermenticuss SCD with is commonly called as Bispan strain has been appropriately used for the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine. In this study, the identification and characterization of Bispan strain was done using SEM observation, API 50CHB kits, isoprenoid quinone analysis, and fatty acid analysis. These results suggest that Bispan strain is very similar to Bacillus subtilis.

• 한국미생물·생명공학회지
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• 제19권6호
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• pp.561-567
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• 1991

Identification of the Actinomycetes isolate strain No. 497 producing an actinomycin antibiotic MT-497 was performed by ISP and chemotaxonomic methods. The strain Nu. 497 formed various shapes of sclerotia and smooth surface spore. Menaquinone MK-9 ($H_6, H_8$) and iso-, anteiso-branched $C_{15}C_{17}$ fatty acids were detected from whole cell extract. The wall chemotype of stram No. 497 was decided as wall chemotype I from the analysis of DAP isomer, peptidoglycan type and sugar pattern. From these morphological, chemotaxonomic characteristics and analysis of various physiological characteristics. the strain No. 497 was identified as Streptomyces nigrifaciens.

• 한국구조물진단유지관리공학회 논문집
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• 제18권2호
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• pp.64-73
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• 2014

본 연구에서는 철골 골조 구조물의 안전성 모니터링을 위하여 계측한 변형률을 통해 구조물에 작용한 하중을 식별하는 알고리즘을 제안한다. 기존의 시스템 식별 연구에서 구조물의 강성 등을 변수화한 것과는 다르게, 본 연구에서는 구조물에 작용한 하중과 이로 인해 구조물에 발생하는 변형률 간의 관계를 행렬로 정의하고, 이 행렬 및 작용한 하중을 변수화 한다. 계측한 변형률과 변수를 통해 추정한 변형률 사이의 차이를 오차함수로 설정하고 이를 최소화시키기 위해 최적화 알고리즘 중 하나인 유전자 알고리즘을 적용한다. 구해진 변수와 계측 변형률을 통해 작용한 하중을 식별하고 구조물의 하중 변화 시 미계측 지점의 응답을 추정한다. 본 연구에서 제안하는 하중 식별 알고리즘을 검증하기 위해 3차원 철골 골조 구조물의 정적 가력 실험을 수행하였고, 계측한 변형률을 통해 가해진 하중을 낮은 오차 수준으로 식별할 수 있었다. 또한, 하중 조건 변화 시, 계측한 변형률을 통해 모니터링 대상이 되는 미계측 지점의 변형률을 0.17~3.13%의 오차 범위로 추정하였다. 본 연구가 제안하는 식별법이 철골 구조물의 보다 현실적인 안전성 모니터링에 효과적으로 적용될 것으로 기대된다.

• Smart Structures and Systems
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• 제23권3호
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• pp.263-278
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• 2019

Moving train load parameters, including train speed, axle spacing, gross train weight and axle weights, are identified based on strain-monitoring data. In this paper, according to influence line theory, the classic moving force identification method is enhanced to handle time-varying velocity of the train. First, the moments that the axles move through a set of fixed points are identified from a series of pulses extracted from the second derivative of the structural strain response. Subsequently, the train speed and axle spacing are identified. In addition, based on the fact that the integral area of the structural strain response is a constant under a unit force at a unit speed, the gross train weight can be obtained from the integral area of the measured strain response. Meanwhile, the corrected second derivative peak values, in which the effect of time-varying velocity is eliminated, are selected to distribute the gross train weight. Hence the axle weights could be identified. Afterwards, numerical simulations are employed to verify the proposed method and investigate the effect of the sampling frequency on the identification accuracy. Eventually, the method is verified using the real-time strain data of a continuous steel truss railway bridge. Results show that train speed, axle spacing and gross train weight can be accurately identified in the time domain. However, only the approximate values of the axle weights could be obtained with the updated method. The identified results can provide reliable reference for determining fatigue deterioration and predicting the remaining service life of railway bridges.

• Structural Engineering and Mechanics
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• 제63권2호
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• pp.251-257
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• 2017

A local damage identification method using measured structural static displacement is proposed in this study. Based on the residual force vector deduced from the static equilibrium equation, residual strain energy (RSE) is introduced, which can localize the damage in the element level. In the case of all the nodal displacements are used, the RSE can localize the true location of damage, while incomplete displacement measurements are used, some suspicious damaged elements can be found. A model updating method based on static displacement response sensitivity analysis is further utilized for accurate identification of damage location and extent. The proposed method is verified by two numerical examples. The results indicate that the proposed method is efficient for damage identification. The advantage of the proposed method is that only limited static displacement measurements are needed in the identification, thus it is easy for engineering application.