Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Optimal Production of Thermostable Alkaline Phosphatase from Thermus caldophilus GK24

Thermus caldophilus GK24로부터 내열성 alkaline phosphatase의 최적생산

  • Kim, You-Jin (Department of Genetic Engineering, Sung Kyun Kwan University) ;
  • Chun, Myung-Sook (Department of Genetic Engineering, Sung Kyun Kwan University) ;
  • Kim, Hyun-Kyu (Department of Genetic Engineering, Sung Kyun Kwan University) ;
  • Kwon, Suk-Tae (Department of Genetic Engineering, Sung Kyun Kwan University)
  • 김유진 (성균관대학교 유전공학과) ;
  • 전명숙 (성균관대학교 유전공학과) ;
  • 김현규 (성균관대학교 유전공학과) ;
  • 권석태 (성균관대학교 유전공학과)
  • Published : 1995.10.31
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Abstract

Thermus caldophilus GK24 was selected as sources of thermostable alkaline phosphatase from a survey of extreme thermophile. T. caldophilus GK24 was tested for production of alkaline phosphatase by addition of various concentration of sodium glutamate, bactotryptone, glucose and yeast extract to basal salts. Sodium glutamate was found to be effective for the alkaline phosphatase induction. The optimal induction medium for production of alkaline phosphatase involves the addition of 0.3% sodium glutamate, 0.2% bactotryptone and 0.5% glucose to basal salts. The activity of the enzyme in optimal induction medium increased nearly 6-fold/ml than basal medium and 27.5-fold/ml than standard medium. T. caldophilus GK24 alkaline phosphatase was found to be inducible. When starved of inorganic phosphate, T. caldophilus GK24 produces the enzyme alkaline phosphatase. The addition of inorganic phosphate to growth medium had a repressive effect on enzyme synthesis.

내열성 alkaline phosphatase의 탐색을 위해 극도호열균중에서 Thermus caldophilus GK24 균주를 선정하였다. 이 균주를 이용하여 basal salts에 sodium glutamate, bactotryptone, glucose 및 yeast extract를 첨가시킨 배지에서 alkaline phosphatase 생산을 검토하였다. 그 결과 sodium glutamate가 alkaline phosphatase 유도에 효과적인 것으로 판명되었다. Alkaline phosphatase 생산을 위한 최적유도용 배지는 basal salts에 0.3% sodium glutamate, 0.2% bactotryptone, 0.5% glucose를 첨가한 것으로 효소활성은 기본배지보다 약 6배, 표준배지 보다는 약 27.5배 증가하였다. T. caldophilus GK24 alkaline phosphatase는 유도효소로 판명되었다. 무기인산 결핍시에 효소가 생산되며, 생육배지에 무기인산을 첨가하면 효소합성에 저해효과가 있었다.

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