Influence of Growth Environment of Anther - Donor Plant and Chilling treatments to Flower Bud on Haploid Plantlets Production in Anther culture of Nicotiana tabacum L.

연초 약배양시 Anther-donor 식물체의 생육조건 및 약의 저온처리가 반수체 출현빈도에 미치는 영향

The present experiments were conducted to investigate some of the factors affecting the number of haploids derived from anther culture of Nicotiana tabacum. Anther - donor plants grown under controlled environment room at 3$0^{\circ}C$ yielded more haploid than room at 18, 25 and 26-22-18$^{\circ}C$ in anther culture. Donor plants starved of fertilizer yielded more haploids as compared to those of the well fed with fertilizer in anther culture. Pretreatment of exercised flower bud at 5$^{\circ}C$ was shown to be more effective in anther culture than pretreatment at 7 and 1$0^{\circ}C$, and the optimum temperature and period of pretreatment were 4 or 6 days at 5$^{\circ}C$.