Long-term Colchicine Prophylaxis on Operative Adhesion Formation in Embryo Transfer Donor Ewes and the Cytogenetic Evalution of Therapy

Embryo Transfer Donor Ewe에 생기는 수술상의 Adhesion 형성에 대한 장기간의 Colchicine 치료와 그에 따른 세포유전학적 분석

Thirty ewes received typical trauma to their oviducts and uterine horns from surgical embryo collection procedures. Ten percent Dexamethasone was used as an irrigant on the exposed abdominal tissue prior to closing the incision. The treatment group received 17mg colchicine Om! lewe) and the control group was administered a 1.0ml placebo(PSS). Fifteen ewes that were initially treated with 17mg /im colchicine showed acute colchicine toxicity within 2-5 days after initial treatment and were removed from the study. Due to acute colchicine toxicity at 17mg, the colchicine level was lowered to 8, 4 and 2mg(4 ewes/group). Treatments consisted of daily injections of colchicine. One ewe in the 8mg group developed toxicity on day 5. Therefore, ewes were then administered colchicine every other day from day 6 to day 14 postsurgeryat 4 and 2 mg. the second laparotomy was performed 9 weeks after first treatment. Following second laparotomy, the treatment group(n=5) received 4 mg colchicine every day for 14 days and there was no clinical symptoms of colchicine toxicity. The third laparotomy was performed by the same operators 5 weeks after final treatment and the adhesions scored. Adhesion grading was based on a scale of 0-4, with 4 being the most severe. The results of adhesion grading(> 3) at second laparotomy were not significantly different(P>0.05)between the two groups. Adhesion formation observed at third laparotomy showed a reduced, but not significant reduction (P>0.05) in the colchicine-treated ewes when compared with the controls. Ten ewes(5 control and 5 treatment)were examined cytogenetically by bone marrow analysis five days post-treatment. There was no difference(P>0.05)in the incidence of numerical or structural aberrations between the two groups.

30마리의 암양에게 surgical embryo collection과정을 통해 oviduct와 uterine horn에 trauma를 생성시켰다. 수술시 절개부위를 봉합하기 직전 노출된 abdominal tissue에 irrigigant로 10% dexamethasone을 사용하였다. 처리군에게는 17mg의 colchicine(1ml/ewe)를 투여했으며, 대조군에게는 1.0ml의 placebo를 처리하였다. 처음 17mg/im의 colchicine를 처리한 15마리 양은 colchicine독성증세를 2∼5일 내에 보였기 때문에 본 연구에서 제외되었다. 17mg에서의 colchicine독성 때문에 colchicine수준은 8, 4 그리고 2mg으로 낮추어졌다. 8mg group에 있던 또 하나의 양은 5일째에 독성증세를 보였기 때문에 나머지 양들은 4와 2mg의 수준으로 이틀에 한번씩 처리되었다. 두 번째 laparotomy는 첫 번째 처리로부터 9주후에 실시되었다. 두 번째 laparotomy후 처리군은 4mg의 colchicine을 이틀에 한번씩 14일 동안 처리되었는데 아무런 독성증세를 나타내지 않았다. 세 번째 laparotomy는 마지막 처리 5주후에 실시되었고 adhesion score로 계산하였다. Adhesion grading은 0∼4의 분포에 근거하여, 4는 가장 심한 adhesion을 나타낸다. 두 번째 laparotomy 결과 adhesion grading( 3)은 두 group 사이에 큰 차리가 없었다(P>0.05). 세 번째 laparotomy결과는 처리군에서 약간 낮은 수치를 보였지만, 통계적으로 두 grouprks에는 큰 차이는 없었다(P>0.05). 10마리의 양(5마리는 대조구, 5마리는 처리구)들은 처리후 5일경에 bone marrow analysis를 통해 cytogenetically분석되었다. 두 grouprks 염색체수와 구조에 있어서 차이가 없었다(p>0.05).