# 톡소플라즈마원충(原蟲)(RH주(株))의 5년간 동결보존시험

• 전영 (가축위생연구소) ;
• 장환 (가축위생연구소)
Toxoplasma gondii frozen by the method of very slow cooling ($-30^{\circ}C/3hrs.{\rightarrow}\;-60^{\circ}C/lhr.{\rightarrow}\;-196^{\circ}C$ of liquid nitrogen)were inoculated mice after storage in the liquid nitrogen for 5 years. The results obtained were as follows; 1. Toxoplasma gondii was detected in mice 4~5th day after intraperitoneal inoculation of organisms stored in the liquid nitrogen once a year for 5 years. 2. Toxoplasma gondii could be preserved longer than 5 years in liquid nitorgen being evidenced by the in vivo test.