Journal of Microbiology and Biotechnology
- Volume 3 Issue 2
- /
- Pages.91-94
- /
- 1993
- /
- 1017-7825(pISSN)
- /
- 1738-8872(eISSN)
Nucleotide Sequencing Analysis of a Gene Coding for 3-Isopropylmalate Dehydrogenase of Kluyveromyces fragilis
- Hong, Soon-Duck (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
-
Kim, Jong-Guk
(Department of Microbiology, College of Natural Sciences, Kyungpook National University)
;
- Lee, Dong-Sun (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
- Woo, Ju-Hyung (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
- Lee, Sang-Yong (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
- Published : 1993.06.01
Abstract
A 3-isopropylmalate dehydrogenase (3-IPMD) gene was cloned from Kluyveromyces fragilis. pJK104 could complement Escherichia coli leuB and Saccharomyces cerevisiae leu2 auxotrophs. The coding region was subcloned and the nucleotide sequence was determined. A 1.8 Kb EcoRI/SphI fragment of pJK104 subcloned in pUC18 could still complement the leuB mutation. An open reading frame of 1164 bp that corresponds to a polypeptide of 387 amino acids was found in the cloned fragment. The homology between the 3-isopropylmalate dehydrogenase of S. cerevisiae and that of K. fragilis was 68.13％ in nucleotides.