Journal of Microbiology and Biotechnology
- Volume 3 Issue 2
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- Pages.86-90
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- 1993
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Cloning and DNA Sequence of Carboxymethylcellulase (CMCase) Gene from Cellulomonas sp. YE-5
- Her, Song (Department of Food Engineering, Yonsei University) ;
- Kim, Dong-Seob (Department of Food Engineering, Yonsei University) ;
- Choi, Sun-Jin (Department of Food Engineering, Yonsei University)
- Published : 1993.06.01
Abstract
CMCase positive clones were screened from Cellulomonas sp. YE-5 and named pCE1, pCE2 and pCE3. Among the positive clones pCE1 was used for this study, because it has the smallest insert and the highest CMCase activity among the 3 clones, and its nucleotide sequence was determined. The CMCase gene in pCE1 was composed of 1071 bp of nucleotides coding 357 amino acids. Computer analysis showed that the pCE1 has 65% sequence homology with the endoglucanase from Cellulomonas fimi.