Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

중금속 카드뮴의 세포독성에 관한 연구

  • 정연태 (원광대학교 환경과학연구소) ;
  • 박승택 (원광대학교 환경과학연구소) ;
  • 문연자 (원광대학교 환경과학연구소) ;
  • 한두석 (원광대학교 환경과학연구소) ;
  • 소진탁 (원광대학교 환경과학연구소)
  • Published : 1993.06.01
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Abstract

The present study was carried out to evaluate the cytotoxicity of cadmium on cultured rat fibroblasts. The colorimetric assays of neutral red and tetrazolium MTT, the lactatedehydrogenase activity, the amounts of total protein, the rate of DNA synthesis, the amounts of unscheduled DNA synthesis, the frequency of sister chromatid exchange, the releasing rate of intracellular calcium, and light and electron microscopic studies were performed on cultured rat fibroblasts maintained in the media containing various concentrations of cadmium. The results were as follows: The neutral red(NR) and MTT values were decreased dose-dependently by cadmium, and the NR90, NR50, MTT90 and MTT50 values of cadmium were 0.2mM, 21.5mM, 1.0Mm and 60.0Mm, respectively.

Keywords

References

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