Archives of Pharmacal Research
- 제16권4호
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- Pages.295-299
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- 1993
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Human Renal Dipeptidase from Kidneys of Renal Stome Patients: Partial Purification
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Park, Haeng-Soon
(College of Pharmacy, Chonnam National University) ;
- Kim, Doh-Ha (College of Pharmacy, Chonnam National University) ;
- Hyun-S.Ellen-Kwark (Pathology Department, Westchester County Medical Center) ;
- Park, Sung-Kwang (Department of Internal Medicine, Medical School, Chonbuk National University) ;
- Kang, Sung-Kyew (Department of Internal Medicine, Medical School, Chonbuk National University) ;
- Chung, Byung-Ho (College of Pharmacy, Chonnam National University) ;
- Yoo, Gyrung-Soo (College of Pharmacy, Chonnam National University)
- 발행 : 1993.12.01
초록
Human renal dipeptidase (RDPase) was purified from surgically removed kdneys of renal stone aptients by affinity chromatography using its specific inhibitor, cilastain, as the ligand. The partial purified RDPase of 6 mg exhivited specific activity of 99.4 unit/mg with 2, 029 fold purification. it was composed of a slow moving major band (96%) and a fast moving minor band (4%). The minor band was not a contaminant as it showed a dipeptidase-specific activity. The kinetic parameters determined with glycyldehydrophenylalanine (Gdp) as synthetic substrate were Vmax,