Archives of Pharmacal Research
- 제16권4호
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- Pages.289-294
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- 1993
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Binding of Sanjoinine-A (Frangufoline) to Calmodulin
- Han, Yong-Nam (Natural Products Research Institute, Seoul National University) ;
- Kim, Geum-Yi (Natural Products Research Institute, Seoul National University) ;
- Hwang, Keum-Hee (Natural Products Research Institute, Seoul National University) ;
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Han, Byung-Hoon
(Natural Products Research Institute, Seoul National University)
- 발행 : 1993.12.01
초록
A binding protein of radio-labeled sanjoinine-A (fangufoline) in rat brain cytoplasm was investigated, using an equilibrium dialysis technique. The labeled agent was bound to the cytosol fraction with two distinctly different types of sets in calclum ion-dependent manner. The bound protein was identified as calmodulin by dgel filtration of the sanjoinine-A bound cytosol fraction on a Sephadex G-75 column. Calmodulin was bound to sanjoinine-A bound at two sets of binding sites in the calculated as two at high affinity sites