Archives of Pharmacal Research
- Volume 16 Issue 2
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- Pages.155-157
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- 1993
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
DNA Transfection in SK-N-BE(2)C Human Neuroblastoma Cells
Abstract
DNA transfection conditions were investigated by calcium phosphate-DNA co-precipitation in SK-N-BE(2)C human neuroblastoma cells. The DNA plasmid of TH2400CAT was used in which rat tyrosine hydroxylase gene was inserted into chloramphenicol acetyltransferase reporter gent. The transfection efficiency was 25-30% and the method was simple and reproducible. So, the method will be a good tool for transient transfection analysis.
Keywords
- Transfection analysis;
- Human neuroblastrma cell;
- Tyrosine hyroxylase;
- Chloram-phenicol acetyltrasferae assay