Production of Anthraquinone Derivatives by Rubia cordifolia var. pratensis Transformed by Agrobacterium spp

Agrobacterium으로 형질전환시킨 갈퀴꼭두선이의 세포배양에 의한 천연염료생산

The cells of Rubia cordifolia var. pratensis were transformed by Agrobactrium tumefaciens strain 11157. Surface-sterilized young leaves and stems of the plants were cocultivated with bacterial suspensions. Crown galls induced from stems were cultured with variation of culturing conditions and compared with untransformed cells. The growth rates and production of anthraquinone pigments of cells were remarkably improved by transformation. Furthermore, hairy roots were induced by inoculation or cocultivation with Agrobacterium rhizogenes strains.