Laboratory Evaluation of Entomopathogenic Nematodes, Steinernema carpocapsae and Heterorhabditis bacteriophora against Some Forest Insect Pests

곤충 병원성 선충 Steinernema carpocapsae와 Heterorhabditis bacteriophora를 이용한 삼립해충의 방제

The pathogenicity of entomopathogenic nematodes Sfeinernema earpocapsae and Heferorhabditis baeferiophora was evaluated against forest insect pests, alder leaf beetle Agelastiea eoerulea, pellucid zygaenid Pryeria siniea, and box-tree pyralid Glyphodes perspeetalis. Alder leaf beetle larvae were exposed to S. earpocapsae at concentration of 0, 25, 50, and 100 nematodes and to H. baeferiophora at concentration of 0, 10, 20, and 40 nematodes per larva on alder leaves. Mortalities of 1st instar lavae were 85.4 $\pm$ 4.1-100%, 2nd instar larvae 80.0 $\pm$ 5.8-100%, and 3rd instar larvae 65.0 $\pm$ 10.8-100% in S. earpocapsae and those of 1st instar larvae were 82.5 $\pm$ 6.9-100%, 2nd instar larvae 77.5 $\pm$ 4.7-100%, and 3rd instar larvae 55.0 $\pm$ 13.5-100% in H. baeferiophora treatment. When pellucid zygaenid larvae were exposed to S. earpocapsae at concentration of 0, 5, 10, 20, 40, and 80 nematodes and to H. baeteriophora at concentration of 0, 2, 5, 10,20, and 40 nematodes per larva, mortalities were 98.9 $\pm$ 1.1-100% in S. earpocapsae and 26.7 $\pm$ 5.1-74.5 $\pm$ 6.2% in H. baeferiophora. The mortalities of box-tree pyralid larvae were 97.8 $\pm$ 1.5-100% in S. earpocapsae treated with concentration of 0,20,40, and 80 nematodes per larva and those were 92.0 $\pm$ 6.2-98.9 $\pm$ 1.1 % in H. baeferiophora treated with con'||'&'||'not;centration of 0, 10, 20, and 40 nematodes per larva.

식엽성 삼림해충인 오리나무잎벌레와 사철나무알락명나방, 회양목명나방에 대한 곤충병원성 선충, Steinernema carpocapsae와 Heterorhabditis bacteriophora의 살충력을 알아보기 위하여 실내 검정한 결과 선충의 조유, 농도, 기주에 따라 차이는 있었으나 높은 치사력을 보았다. 오리나무잎벌레인 경우 S.carpocapsae를 유충 한 마리당 25~100마리/ml 농도로 엽상에 처리했을 때 1령충 85.0$\pm$4.1~100%, 2령충 80.0$\pm$5.8~100%, 3령충 65.0$\pm$10.8~100%의 치사율을 보였으며, H. bacteriophora 10~40마리/ml 처리에서는 1령충 82.5$\pm$6.9~100%, 2령충 77.5$\pm$4.7~100%의 치사율을 나타내었다. 사철나무 알락명나방은 유충 gs마리당 S. carpocapsae 5~80마리/ml 처리에서 98.9$\pm$1.1~100%, H. bacteriophora 2~40마리/ml 처리에서는 26.7$\pm$5.1~74.5$\pm$6.2%의 치사율을 나타내었으며, 회양목명나방은 유충한마리당 S. carpocapsae 20~80마리/ml 처리구에서 97.8$\pm$1.5~100%, H. bacteriophora 10~40마리/ml 처리구에서 92.0$\pm$6.2~98.9$\pm$1.1%의 살충력을 보였다.