• Korean journal of applied entomology
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• v.30 no.4
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• pp.258-264
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• 1991

Studies were carried out to investigate effects of temperature and photoperiod on diapause induction and of juvenile hormone III and 20-hydroxyecdysone treatment on diapausing adults of the alder leaf beetle, Agelastica coerulea Baly(Chrγsomelidae: Coleoptera). Its life cycle and ovarian development in adults were also observed. The beetle had one year life cycle with egg, larval, pupal and adult periods being 7-10, 19-21, 14-15 days and about 10 months, r respectively. All adults showed a diapause syndrome when the larvae were reared at $20^{\circ}C$ or $25^{\circ}C$ in combination of photoperiods of 16L/8D, 12L/12D, or 8L/16D. Their ovarioles did not s show any development of vitellogenesis before or during diapause and even when exposed at $15^{\circ}C$ after overwintering. When diapausing adults were treated with JH III they resumed feeding and laid several eggs and broke diapause condition temporally. But diapausing adults treated with 20-hydroxyecdysone did not show any response.

• Asian Journal of Turfgrass Science
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• v.11 no.3
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• pp.185-191
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• 1997

Biological control of turfgrass insect pest Blitopertha orientalis, forest insect pests, Agelastica coerulea, Meganola melancholia, and Glyphodes perspectalis,vegetable insect pests, Plutella xylostella and Agrotis segetum were conducted with entonopathogenic fungus, Metarhizium anisopliae J-22 isolated from black pine forest soil in Cheju province. Mortality of B. orientalis larvae was 53.3% at the rate of 3.4 $\times$ 1O 7 conidia /ml. A. coerulea and M melancholia larvae showed 100% mortalities at 9.6 $\times$ 106 conidia /ml and 2.7 $\times$ 10 7 conidia /ml as well. However, G. perspectalis larvae were not dead even at 4$\times$ 1O 7 conidia /ml. On the other hand, M anisopliae J-22 was effective against P. xylostella larvae showing 100% mortality at 4 $\times$ 10 7 conidia /ml. KEy words:Entomopathogenic fungi, Biological control, Metarhizium anisopliae, Pathogenicity,Blitopertha orientalis, Agelastica coerulea, Meganola melancholia, Glyphodes perspectalis,Plutella xylostella, Agrotis segetum.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2002.11a
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• pp.113-113
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• 2002

• Korean Journal of Environmental Agriculture
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• v.5 no.2
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• pp.119-129
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• 1986

Disappearence of the trichlorfon (Dipterex) in the forest, following aerial to control Agelastica coerulea B., were studied by sampling deposits on slide glasses, soils, water, and leaves, and analysing with a gas chromatograph equipped with an electron capture detector. By analysing the amount remained on slide glasses, it was shown that the pesticide was adequately sprayed and nearly all deposit was lost in a day. The amount deposited under the tree was about 1/100 of the amount at an exposed site. Concentration of trichlorfon in creek water was 10 to 100 times as high as the acute toxic level to zooplankton for 6 to 24 hours, The rain could recontaminate the stream water up to the toxic level. Loss rate of trichlorfon from soils showed variations by sampling sites and was generally slower than from slide glasses. Amount deposited on leaves were less than the calculated or expected amount. The loss from leaves were similar to that from soil.

• Microbiology and Biotechnology Letters
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• v.25 no.2
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• pp.159-165
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• 1997

We collected 3,237 plant samples, mainly leaves of various trees, from many provinces in Korea and a total of 1,925 Bacillus thuringiensis isolates were obtained and characterized. The isolates were characterized in terms of crystal morphology, PAGE pattern of the toxin proteins, plasmids pattern, biochemical characteristics, and bioassay. The microscopic observation showed that 49.1% of the isolates have bipyramidal shape crystals, 7.1% of spherical shape crystals, 1.4% of rhomboidal shape crystals, and others have small or amorphous inclusions. The insecticidal activities of the spore-crystal mixtures of isolates were tested against Plutella xylostella, Bombyx mori, Culex pipiens, and Agelastica coerulea. Bioassay showed that 51.3% of the isolates were shown to be active; lepidopteran-specific (44.8%), dipteran-specific(4.9%) and coleopteran-specific (1.6%). The remainder(48.8%) did not show any activity against the insects we tested. Interestingly though, some of these non-active isolates were shown to have bipyramidal crystals. By serotyping 22 isolates of our collection, we found that there are various kind of subspecies such as aizawai, amagiens, canadensis, darmstadiensis, galleriae, finitimus, kurstaki, morrisoni and neoleonensis, and three isolates have been classified into a new serotype, H49, and one of them, the type strain, named subsp. muju. From this study it was found that phylloplane is a good source for the isolation of Bacillius thuringiensis, and Bacillus thuringiensis is distributed widely in Korea.

• Korean journal of applied entomology
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• v.36 no.4
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• pp.351-356
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• 1997

Biological control of forest insect pests, Agelastica coeruleci, Meganola n~elancholia,a nd Glyphodes perspectalis, turfgrass insect pest, Blitoperfhu orientr~lis,v egetable insect pests, Plutella xylostella and Agrotis segetun~ with entomopathogenic fungus, Beciuveria hassinna GY 1 - 17 isolated from rice paddy of Yangsan in the southern part of Korea were investigated. Mortalities of A. coeruleu and P. ~ylo.~rellluar vae were 100% at the rate of 7.0 and 2.0 x 107conidia/ml after 7 and 5 days and those of M. melancholia were 66.7 - 100% at the rate of 0.03875-3.1 X 107conidia/ml. However, G. perspectulis was not affected at the rate of 2.0 x lo7 to X I04conidia/ml. And mortalites of B. orientcilis and A. segetum larvae were 46.7% at 3.7 x 107conidia/ml and 63.3% at 2.5 X 107conidia/ml.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.5
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• pp.59-69
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• 2003

Most denuded mountain areas in Korea were completely stabilized by the successful work of the 1st and 2nd 10-year Forest Development Plans which targeted the reforestation of denuded forest lands. The objectives of this study are (1) to estimate the depth of organic horizon in the soil profile, (2) to investigate the change of vegetation structure, (3) to estimate the change of biomass in the erosion controlled and rehabilitated mountain areas with the passage of time. This study was carried out as the 5th times. The first study began in the year of 1985, the second study was in the year of 1988, the third study was in the year of 1992, 4th was in the year of 1998 and 5th was in the year of 2002. The first study started in the study sites which elapsed 9 years after erosion control works. The results of the study were as follows : The increase rate of soil thickness was estimated to $Y_{(cm)}=2.906log_{(yr)}-3.2476(r^2=0.917)$ during 26 years after erosion control works. The important value of pines decreased to 14.7% on upper layer. But, the important value of alders. which did not plant on erosion control work increased to 27.1%. The decrease of whole crown projection indicates that pines. and alders were heavily injured by pine leaf gall midge in the year of 1993, 1995 years and Agelastica coerulea Baly in the year of 1986, 1987 years at Yoju-gun. The young growth of pines and alders not appeared on the soil surface which elapsed 26 years after erosion control works. On the lower layer, oaks occupied over 50% in I.V, RD, RC, RF. In process of years, the increase of biomass estimated to be $Y_{(t/ha)}={0.7505X_{(yr)}}^{1.6335}\;(r^2=0.9712)$ for 26 years after erosion control works.

• Journal of Korean Society of Forest Science
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• v.81 no.4
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• pp.303-309
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• 1992

This study eras carried out to investigate the ecological changes on the plant community at erosion controlled area in Yoju-gun, Kyonggi-do. The results of the study were as follows : 1. The depth of organic matters in the soil profile from 4 to 14 years was estimated to be $Y_{(cm)}=0.436X_{(yr)}-0.931$(r=0.978), and 02 layer was $Y_{(cm)}=0.339_{(yr)}-0.931$ (r=0.954). 2. On upper plant layer. Alnus spp. was decreased, on an average, 32% of relative coverage, 12.4% of relative density and 16.8% of relative frequency in each plot for 3 years, as this area was damaged by Agelastica coerulea Baly for 2-3 years. 3. On upper plant layer, relative coverage of Pinus spp. was increased after 6, 7 years in erosion controlled area and was taked the highest plant (more then 40%) from 10 to 14 years. 4. On lower plant layer, relative density of Quercus ssp. (these species has not been planted and sown on erosin controlling) was increased all the plot. 5. The increase of the whole crown projection area was estimated to be $Y_{(m^2)}=18.020X_{(yr)}+18.834$(r=0.954) 6. The biomass was estimated to be 14.88t/ha on elapsed 6 years, 22.84t/ha on 8 years, 35.08t/ha on 10 years, 47.80t/ha on 12 years, 58.13t/ha on 14 years.

• Korean Journal of Environmental Agriculture
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• v.8 no.1
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• pp.17-29
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• 1989

This study was conducted to evaluate the behavior in the water and the impact on aquatic organisms following aerial application of two insecticides in the forest, cyfluthrin and trichlorfon, to control the alder leaf beetle. As active ingredients, 25g of cyfluthrin and 536g of trichlorfon per ha were diluted seperately into 30L of tap water, and applied with a helicopter to the study areas. A model stream study was also conducted in a stream located adjacent to the study area in order to confirm the impact of insecticides on aquatic invertebrates. Cyfluthrin residues in water were $0.62{\mu}g/L$ (1st. application) and $78{\mu}g/L$ (2nd application) immediately after spraying. and decreased, to a non-detectable level after one day, while trichlorfon residue increased to $30.7{\mu}g/L$ one day after spraying and fluctuated for 22th day depending on precipitation after spraying. Cyfluthrin application rapidly increased the number of some drifting aquatic invertebrates during 24-hour period immediately after spraying, but had no effects on the other aquatic organisms such as fish and zooplankton. The largest increase in the number of drifting organisms following application of cyfluthrin was shown by Ephemeroptera, and followed by Trichoptera, Coleoptera, and Diptera. However, trichlorfon little affected the number of drifting aquatic invertebrates and zooplankton population.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.94-105
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• 1993

The genus Cordyceps known as an insect parasite forms a sclerotium in insect bodies and then produces perithecia on the single or multiple stromata produced from sclerotium. Collected Cordyceps were identified into 5 species: Cordyceps militaris, C. nutans, Cordyceps sphecocephala, Isaria japonica, and Torrubiella sp. The fruit bodies of Cordyceps in petri-dish cover were fixed by tape and put the lid on water agar plates to isolate these collected Cordyceps. The germinated spores were transferred from water agar to Potato dextrose agar(PDA) after six hours. Mycelial growth of C. nutans and C. militaris was the most successful on Hamada media and was also good on Complete media and PDA. Mannose as a carbon source was good for two species and Glutamic acid as a nitrogen source was satisfactory to C. militaris and Asparagine gave a good result to C. nutans. C. militaris and C. nutans showed similar mycelial growth rate on the media that contained thiamine-HCI, biotine or nicotinic acid as a vitamine. When conidia of C. nutans were inoculated to insects, mortality was high in Artogeia napi L, Hemiptera, Plutella xylostella and 50% in Orthoptera, 12% in Acantholyda posticalise M, but not Agelastica coerulea B. in Aphididae, C. nutans was collected from only Hemiptera in nature, but killing effect on other insects was proved. Mycelial growth and fruit-body formation were good on the media that consist of rice powder 5g, wheat flour 5g, water 100ml, but formed fruit-body was not complete stromata but a mass of conidia according to results of observing microscope.