The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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N-Terminal Sequence of Soybean $\beta$- Amylase

대두 $\beta$- Amylase의 N-말단 아미노산 배열

  • 지의상 (신흥전문대학 식품영양과) ;
  • 김관묵 (김천전문대학 식품영양과, 중앙대학교 식품가공학과) ;
  • 김준평
  • Published : 1991.10.01
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Abstract

The blocked N-terminus and N-terminal sequence of soybean B-amylase were aetermined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50$\times$2 column(1X5cm) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptlde. The N-terminal 7 amino acid sequence of soybean B-amylase was deduced to be acetyl-Ala-Thf-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.

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