Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 18 Issue 3
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- Pages.286-291
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- 1990
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Purification and Characterization of Antibiotics KG-1167A & KG-1167B Produced by Clostridium sp.
Clostridium sp.가 생산하는 항생물질 KG-1167A와 KG-1167B의 정제 및 특성
Abstract
The antibiotics KG-1167A and KG-1167B were isolated from the fermentation broth of the bacterial strain KH-1167, identified as Cloetridium sp. The individual antibiotics, KG-1167A & KG-1167B were separated and purified by solvent extraction followed by silica gel column chromatography. These antibiotics showed antibacterial activities against broad spectrum of gram positive and gram negative bacteria. The physico-chemical properties and UV spectra suggest that they possess aromatic moiety in their structures.
순수분리된 2,200여주의 혐기성 세균으로부터 선별된 Clostridium sp. KH-1167은 그람양성 및 음성균에 대하여 재현성있는 항균활성을 나타내는 무질을 생산하였다. Solvent extraction, silica gel column chromatography에 의하여 배양액으로부터 항생물질 KG-1167A와 KG-1167B를 부분정제 하였다. 이들은 pH4-11의 범위에서는 비교적 안정하였으나, 온도에 대해서는